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PICM0RG50

Millipore

Millicell® Standing Cell Culture Inserts

pore size 0.4 μm, diam. 30 mm, transparent PTFE membrane, hydrophilic, H 5 mm, size 6 wells, sterile

Synonim(y):

Millicell Cell Culture Insert, 30 mm, hydrophilic PTFE, 0.4 µm, Millicell-CM, cell culture inserts, organotypic inserts, plate inserts

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About This Item

Kod UNSPSC:
41104923
eCl@ss:
32011202
NACRES:
NB.22

Materiały

polystyrene housing
transparent PTFE membrane

Poziom jakości

400

sterylność

ethylene oxide treated
sterile

Właściwości

hydrophilic

producent / nazwa handlowa

Millicell®

opakowanie

pkg of Individually blister packaged

Parametry

50 °C max. temp.

metody

cell attachment: suitable
cell culture | mammalian: suitable
cell differentiation: suitable

Wysokość

5 mm

Średnica

30 mm

rozmiar

6 wells

powierzchnia

4.2 cm2

kolor

transparent membrane, when wetted

Matryca

Biopore™

wielkość porów

0.4 μm

typ wiązania

low binding surface

metoda wykrywania

fluorometric

Warunki transportu

ambient

Opis ogólny

With Millicell inserts, attachment or suspension cells can access media from both their apical and basolateral sides. Cell growth, structure, and function more closely mimic what occurs in vivo. In addition, Millicell inserts make it possible to study both sides of the cell monolayer.

Zastosowanie

  • Cell Attachment, 3D Cell Culture, Cell Growth, Cell Differentiation , Immunocytochemistry
  • Biopore Membrane (hydrophilic PTFE) is ideal for low protein-binding, live cell viewing, and immunofluorescent applications

Cechy i korzyści

  • For high cell viability and superior study of three dimensional explant structure
  • Lower height allows them to fit inside a standard petri dish
  • The Biopore (PTFE) membrane provides high viability—for as long as 40 days—and excellent trans-membrane oxygen transport
  • The membrane is optically clear and optimized for long-term organotypic explant maintenance

Uwaga dotycząca przygotowania

To ensure proper wetting of the membrane from the basolateral side and to mitigate a chance of bubble formation:
  1. Add assay solution or media to the receiver well: 2 mL for 6 well format orequivalent dimensions.
  2. With forceps, place the insert into the receiver well at a 30°-45° angle togradually wet it out from the basolateral side.
  3. Once edges start to wet out, gently lower the insert until it is fully seatedinto the well.

Informacje prawne

BIOPORE is a trademark of Merck KGaA, Darmstadt, Germany
Millicell is a registered trademark of Merck KGaA, Darmstadt, Germany
Ta strona może zawierać tekst przetłumaczony maszynowo.

Kod klasy składowania

10-13 - German Storage Class 10 to 13

Certyfikaty analizy (CoA)

Poszukaj Certyfikaty analizy (CoA), wpisując numer partii/serii produktów. Numery serii i partii można znaleźć na etykiecie produktu po słowach „seria” lub „partia”.

Masz już ten produkt?

Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.

Odwiedź Bibliotekę dokumentów

Ryota Tamura et al.
Human gene therapy, 31(5-6), 352-366 (2020-02-23)
Glioblastoma is the most aggressive brain tumor characterized by diffuse infiltration into the normal brain parenchyma. Neural stem cells are known to possess the tumor-tropic migratory capacity and thus can be used as cellular vehicles for targeted delivery of therapeutic
Keisuke Ikegami et al.
Investigative ophthalmology & visual science, 61(3), 26-26 (2020-03-18)
Elevated IOP can cause the development of glaucoma. The circadian rhythm of IOP depends on the dynamics of the aqueous humor and is synchronized with the circadian rhythm pacemaker, that is, the suprachiasmatic nucleus. The suprachiasmatic nucleus resets peripheral clocks
Abigail K Myers et al.
Development (Cambridge, England), 147(2) (2020-01-10)
The precise migration of cortical interneurons is essential for the formation and function of cortical circuits, and disruptions to this key developmental process are implicated in the etiology of complex neurodevelopmental disorders, including schizophrenia, autism and epilepsy. We have recently
Andrea S Cupp et al.
Biology of reproduction, 68(6), 2033-2037 (2003-02-28)
The first morphological event after initiation of male sex determination is seminiferous cord formation in the embryonic testis. Cord formation requires migration of pre-peritubular myoid cells from the adjacent mesonephros. The embryonic Sertoli cells are the first testicular cells to
P Hardman et al.
Development, growth & differentiation, 35(6), 683-690 (1993-01-01)
Embryonic mouse salivary glands, pancreata, and kidneys were isolated from embryos of appropriate gestational age by microdissection, and were cultured on Biopore membrane either non-coated or coated with type I collagen or Matrigel. As expected, use of Biopore membrane
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Protokoły

Toluidine Blue Staining Protocol for Millicell®

This is a Toluidine Blue Staining protocol.

Protokół barwienia błękitem toluidyny dla wkładów Millicell®

Błękit toluidynowy selektywnie barwi materiał jądrowy i kwaśne składniki tkanek, pomagając w barwieniu histologicznym tkanek bogatych w DNA/RNA.

Microscopic Examination of Samples

This protocol covers 3 modes for the microscopic examination of cell samples.

Indirect Co-culture of Embryonic Stem Cells with Embryonic Fibroblasts

This page covers the indirect co-culture of embryonic stem cells with embryonic fibroblasts.

Zobacz wszystko

Powiązane treści

Narzędzia do pracy z kulturami komórkowymi 3D

Zapoznaj się z naszą ofertą narzędzi i technologii do hodowli komórek 3D dla dowolnej linii komórek 3D lub zastosowania i odkryj organoidy, hydrożele, ULA, mikrodołki lub płytki hydrożelowe i nie tylko.

ECM Coating Protocols for Millicell®

This page covers the ECM coating protocols developed for four types of ECMs on Millicell®-CM inserts, Collagen Type 1, Fibronectin, Laminin, and Matrigel.

Nasz zespół naukowców ma doświadczenie we wszystkich obszarach badań, w tym w naukach przyrodniczych, materiałoznawstwie, syntezie chemicznej, chromatografii, analityce i wielu innych dziedzinach.

Skontaktuj się z zespołem ds. pomocy technicznej