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Merck

I3390

Sigma-Aldrich

IMDM

With sodium bicarbonate, without ʟ-glutamine, liquid, sterile-filtered, suitable for cell culture

Synonim(y):

Iscove′s MDM, Iscoves MDM, Iscove’s Modified Dulbecco’s Medium, IMDM

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About This Item

Numer MDL:
Kod UNSPSC:
12352207
NACRES:
NA.75

Nazwa produktu

Iscove′s Modified Dulbecco′s Medium, liquid, sterile-filtered, With sodium bicarbonate, without L-glutamine, suitable for cell culture, suitable for hybridoma

Poziom jakości

sterylność

sterile-filtered

Formularz

liquid

metody

cell culture | hybridoma: suitable
cell culture | mammalian: suitable

zanieczyszczenia

endotoxin, tested

komponenty

phenol red: yes
L-glutamine: no
glucose: yes

Warunki transportu

ambient

temp. przechowywania

2-8°C

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Zastosowanie

Guilbert and Iscove demonstrated that precursor cells of erythrocytes and macrophages could be cultured in a totally defined serum free medium supplemented with albumin, transferrin, lecithin, and selenium. This medium is a modification of Dulbecco′s Modified Eagle′s Medium (DME) containing selenium, additional amino acids and vitamins, sodium pyruvate, HEPES buffer, and potassium nitrate instead of ferric nitrate. Further studies demonstrated that Iscove′s Medium would support murine B lymphocytes, hemopoietic tissue from bone marrow, B cells stimulated with lipopolysaccharide, T lymphocytes, and a variety of hybrid cells.

Rekonstytucja

Supplement with 0.584 g/L L-glutamine
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Kod klasy składowania

12 - Non Combustible Liquids

Klasa zagrożenia wodnego (WGK)

WGK 1

Temperatura zapłonu (°F)

Not applicable

Temperatura zapłonu (°C)

Not applicable


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Hematological malignancies are characterised by a block in differentiation, which in many cases is caused by recurrent mutations affecting the activity of hematopoietic transcription factors. RUNX1-EVI1 is a fusion protein formed by the t(3;21) translocation linking two transcription factors required
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The Journal of experimental medicine, 147(3), 923-933 (1978-03-01)
Albumin, transferrin, and lipids can replace serum entirely for support of LPS-stimulated murine B lymphocytes in culture. In the presence of these compounds, growth and maturation to IgM and IgG secretion, induced by lipopolysaccharide (LPS), occurs at the same or
S S Ozturk et al.
Journal of biotechnology, 18(1-2), 13-28 (1991-04-01)
A murine hybridoma cell line (167.4G5.3) was cultured in batch mode using IMDM containing different serum concentrations and bovine serum albumin (BSA). Cell growth and death, metabolism and antibody production were studied in these cultures. The cells were more susceptible

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