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D5921

Sigma-Aldrich

DMEM - low glucose

With 1000 mg/L glucose, and sodium bicarbonate, without L-glutamine and phenol red, liquid, sterile-filtered, suitable for cell culture

Synonim(y):

DME, Dulbecco′s Modified Eagle′s Medium - high glucose, DMEM

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About This Item

Numer MDL:
MFCD00217342
Kod UNSPSC:
12352207
NACRES:
NA.75

product name

Dulbecco′s Modified Eagle′s Medium - low glucose, With 1000 mg/L glucose, and sodium bicarbonate, without L-glutamine and phenol red, liquid, sterile-filtered, suitable for cell culture

sterylność

sterile-filtered

Postać

liquid

metody

cell culture | mammalian: suitable

zanieczyszczenia

endotoxin, tested

komponenty

phenol red: no
sodium pyruvate: no
glucose: low
HEPES: no
L-glutamine: no
NaHCO3: yes

Warunki transportu

ambient

temp. przechowywania

2-8°C

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Opis ogólny

Dulbecco′s Modified Eagle′s medium (DME) is a modification of Basal Medium Eagle (BME), which has a four-fold higher concentration of amino acids and vitamins along with additional supplementary components. The first original DME formula was reported to culture embryonic mouse cells comprising 1,000 mg/L of glucose. An alteration with 4,500 mg/L glucose is optimal in cultivating certain cell types. DME has been shown to improve the stability of the medium.

Zastosowanie

Dulbecco′s Modified Eagle′s medium-low glucose has been used to culture:
  • living colon cancer SW620 cells
  • human breast carcinoma cell lines MCF-7(13), KPL and MDA-MB-231
  • mammal and canine derived adipose-derived mesenchymal stem cells

Rekonstytucja

Supplement with 0.584 g/L L-glutamine.

Inne uwagi

Investigators who work with stem cells or cell clones frequently prefer media without phenol red.
This page may contain text that has been machine translated.

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Numer produktu
Opis
Cennik

suplement

Numer produktu
Opis
Cennik

Piktogramy

Exclamation mark
GHS07

Hasło ostrzegawcze

Warning

Zwroty wskazujące rodzaj zagrożenia

H317

Zwroty wskazujące środki ostrożności

P261 - P272 - P280 - P302 + P352 - P333 + P313 - P362 + P364

Klasyfikacja zagrożeń

Skin Sens. 1

Kod klasy składowania

12 - Non Combustible Liquids

Klasa zagrożenia wodnego (WGK)

WGK 3

Temperatura zapłonu (°F)

Not applicable

Temperatura zapłonu (°C)

Not applicable

Certyfikaty analizy (CoA)

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