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Merck

D6434

Sigma-Aldrich

DMEM/F-12

With HEPES, sodium bicarbonate and sodium pyruvate, without ʟ-glutamine and phenol red, liquid, sterile-filtered, suitable for cell culture

Synonim(y):

DME/Nutrient Mixture F-12 Ham, DMEM Hams F12, DME/F-12, 1:1 mixture

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About This Item

Numer MDL:
Kod UNSPSC:
12352207
NACRES:
NA.75

product name

Dulbecco′s Modified Eagle′s Medium/Nutrient Mixture F-12 Ham, With 15 mM HEPES and sodium bicarbonate, without L-glutamine and phenol red, liquid, sterile-filtered, suitable for cell culture

sterylność

sterile-filtered

Postać

liquid

metody

cell culture | mammalian: suitable

zanieczyszczenia

endotoxin, tested

komponenty

L-glutamine: no
phenol red: no
NaHCO3: yes
HEPES: 15 mM
sodium pyruvate: 0.055 g/L
glucose: 3.15 g/L

Warunki transportu

ambient

temp. przechowywania

2-8°C

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Opis ogólny

Pyridoxal, which is an aldehyde, has been replaced with pyridoxine. This modification has been shown to improve the stability of the medium. Suitable for growth of a wide range of cell types in low serum conditions. Absence of phenol red makes this a preferred medium for clonal and stem cell culture.

Zastosowanie

Dulbecco′s Modified Eagle′s Medium/Nutrient Mixture F-12 Ham has been used to culture:

  • bovine endometrial epithelial cells
  • cancer stem cells
  • breast cancer cell line

Rekonstytucja

Supplement with 0.365 g/L L-glutamine
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Piktogramy

Exclamation mark

Hasło ostrzegawcze

Warning

Zwroty wskazujące rodzaj zagrożenia

Zwroty wskazujące środki ostrożności

Klasyfikacja zagrożeń

Skin Sens. 1

Kod klasy składowania

12 - Non Combustible Liquids

Klasa zagrożenia wodnego (WGK)

WGK 3

Temperatura zapłonu (°F)

Not applicable

Temperatura zapłonu (°C)

Not applicable


Certyfikaty analizy (CoA)

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Odwiedź Bibliotekę dokumentów

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Methionine dependency of tumor growth, although not well-understood, is detectable by 11C-methionine positron emission tomography and may contribute to the aggressivity of glioblastomas (GBM) and meningiomas. Cytosolic folate cycle is required for methionine synthesis. Its dysregulation may influence cell reprogramming

Produkty

Mieszanka składników odżywczych F-12 Ham Formulation

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