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Merck

D8437

Sigma-Aldrich

DMEM/F-12

With ʟ-glutamine, HEPES, sodium pyruvate and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture

Synonim(y):

DME/Nutrient Mixture F-12 Ham, DMEM Hams F12, DME/F-12, 1:1 mixture

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About This Item

Numer MDL:
Kod UNSPSC:
12352207
NACRES:
NA.75

product name

Dulbecco′s Modified Eagle′s Medium/Nutrient Mixture F-12 Ham, With L-glutamine, 15 mM HEPES, and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture

Poziom jakości

sterylność

sterile-filtered

Postać

liquid

metody

cell culture | mammalian: suitable

zanieczyszczenia

endotoxin, tested

komponenty

glucose: 3.15 g/L
HEPES: yes
L-glutamine: yes
phenol red: yes
sodium pyruvate: 0.055 g/L

Warunki transportu

ambient

temp. przechowywania

2-8°C

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Opis ogólny

Dulbecco′s Modified Eagle′s medium (DMEM), a regularly used cell culture medium, is a source of energy-rich media that includes glucose, vitamins, and amino acids. A 1:1 mixture of Dulbecco′s Modified Eagle′s medium (DME) and Ham′s F-12 nutrient mixture is commonly used in cell culture research. To compensate for the loss of buffering capacity caused by removing serum, 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) buffer may be added in the formulations at a final concentration of 15 mM.

Zastosowanie

Dulbecco′s Modified Eagle′s Medium/Nutrient Mixture F-12 Ham:
  • has been used as a component in the culture medium to culture primary culture-activated human hepatic stellate cells
  • has been used to culture the cartilage biopsies
  • may be used for the propagation of Cowdria ruminantium

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Kod klasy składowania

12 - Non Combustible Liquids

Klasa zagrożenia wodnego (WGK)

WGK 2

Temperatura zapłonu (°F)

Not applicable

Temperatura zapłonu (°C)

Not applicable


Certyfikaty analizy (CoA)

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Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.

Odwiedź Bibliotekę dokumentów

Yusuke Matsuya et al.
Scientific reports, 9(1), 10365-10365 (2019-07-19)
Insoluble radioactive microparticles emitted by the incident at the Fukushima nuclear power plant have drawn keen interests from the viewpoint of radiation protection. Cs-bearing particles have been assumed to adhere in the long term to trachea after aspirated into respiratory
Shangwu Chen et al.
Biomaterials, 73, 23-31 (2015-09-24)
Preparation of three-dimensional (3D) micropatterned porous scaffolds remains a great challenge for engineering of highly organized tissues such as skeletal muscle tissue and cardiac tissue. Two-dimensional (2D) micropatterned surfaces with periodic features (several nanometers to less than 100 μm) are
Chris M Valdez et al.
Scientific reports, 7(1), 16372-16372 (2017-11-29)
A bipolar (BP) nanosecond electric pulse (nsEP) exposure generates reduced calcium influx compared to a unipolar (UP) nsEP. This attenuated physiological response from a BP nsEP exposure is termed "bipolar cancellation" (BPC). The predominant BP nsEP parameters that induce BPC
Iago Méndez-López et al.
Journal of hypertension, 35(8), 1594-1608 (2017-04-14)
It has been suggested that Ca entry through store-operated Ca channels (SOCs) is regulated by a dynamic interplay between the endoplasmic reticulum Ca stores and the mitochondria. These relationships drive the activation and inactivation of SOCs, yet it remains unclear
Višnja Stepanić et al.
Antioxidants (Basel, Switzerland), 8(4) (2019-04-10)
Chalcones are polyphenolic secondary metabolites of plants, many of which have antioxidant activity. Herein, a set of 26 synthetic chalcone derivatives with alkyl substituted pyrazine heterocycle A and four types of the monophenolic ring B, were evaluated for the potential

Produkty

Mieszanka składników odżywczych F-12 Ham Formulation

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