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Merck

D4522

Sigma-Aldrich

Deoxyribonucleic acid from calf thymus

Type XV, Activated, lyophilized powder

Synonim(y):

Calf Thymus DNA, DNA from calf thymus, Thymonucleic acid

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About This Item

Numer CAS:
Numer WE:
Numer MDL:
Kod UNSPSC:
41106310
eCl@ss:
32160414
NACRES:
NA.52

klasa czystości

for molecular biology

Poziom jakości

typ

Type XV

Postać

lyophilized powder

temp. przechowywania

−20°C

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Opis ogólny

High quality double-stranded template DNA isolated from the thymus of male and female calves.

Zastosowanie

Calf thymus DNA is exceptionally useful as a substrate for DNA polymerase assays, in the amplification of very long fragments and as a carrier DNA for precipitations. It was used as a standard to determine DNA content in gels and for DNA quantification by fluorescent assay.
Calf thymus DNA is exceptionally useful as a substrate for DNA polymerase assays, in the amplification of very long fragments and as a carrier DNA for precipitations.

Cechy i korzyści

• No nuclease activity detected after 16 h at 37 °C incubation
• High quality template DNA

Ilość

One mg DNA is approx. 20 A260 units.

Uwaga dotycząca przygotowania

Prepared from highly polymerized calf thymus DNA and nicked with DNase using the method of Aposhian and Kornberg.

Rekonstytucja

Dissolve the DNA gently rocking overnight to a final concentration of 1mg/ml in molecular biology grade water.
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produkt powiązany

Numer produktu
Opis
Cennik

Kod klasy składowania

11 - Combustible Solids

Klasa zagrożenia wodnego (WGK)

WGK 3

Temperatura zapłonu (°F)

Not applicable

Temperatura zapłonu (°C)

Not applicable

Środki ochrony indywidualnej

Eyeshields, Gloves


Certyfikaty analizy (CoA)

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Masz już ten produkt?

Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.

Odwiedź Bibliotekę dokumentów

Claudia Gomes et al.
Life science alliance, 4(11) (2021-09-11)
High levels of autoimmune antibodies are observed in COVID-19 patients but their specific contribution to disease severity and clinical manifestations remains poorly understood. We performed a retrospective study of 115 COVID-19 hospitalized patients with different degrees of severity to analyze
Parul Natvar Patel et al.
Tissue engineering, 11(9-10), 1498-1505 (2005-11-02)
The ultimate goal of this research is to develop an injectable cell-scaffold system capable of permitting adipogenesis to abrogate soft tissue deficiencies resulting from trauma, tumor resection, and congenital abnormalities. The present work compares the efficacy of photopolymerizable poly(ethylene glycol)
Anastasia S Singatulina et al.
Cell reports, 27(6), 1809-1821 (2019-05-09)
PARP-1 synthesizes long poly(ADP-ribose) chains (PAR) at DNA damage sites to recruit DNA repair factors. Among proteins relocated on damaged DNA, the RNA-binding protein FUS is one of the most abundant, raising the issue about its involvement in DNA repair.
Enzymatic synthesis of deoxyribonucleic acid. IX. The polymerase formed after T2 bacteriophage infection of Escherichia coli: a new enzyme.
H V APOSHIAN et al.
The Journal of biological chemistry, 237, 519-525 (1962-02-01)
Benjamin D Dickson et al.
Molecules (Basel, Switzerland), 24(8) (2019-04-24)
Poly(ADP-ribose)polymerase (PARP) inhibitors (PARPi) have recently been approved for the treatment of breast and ovarian tumors with defects in homologous recombination repair (HRR). Although it has been demonstrated that PARPi also sensitize HRR competent tumors to cytotoxic chemotherapies or radiotherapy

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