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S0902

Sigma-Aldrich

SSC Buffer

for Northern and Southern blotting, powder blend

Synonim(y):

Saline-sodium citrate buffer

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About This Item

Kod UNSPSC:
41105319
NACRES:
NA.25

Formularz

powder blend

pH

6.8-7.2 (20-25 °C, 263 mg/L in water)

rozpuszczalność

water: 263 mg/mL, clear, colorless

obecność zanieczyszczeń

DNAse, none detected
Endonuclease, none detected
Exonuclease, none detected
NICKase, none detected
RNAse, none detected

temp. przechowywania

room temp

Opis ogólny

SSC Buffer 20x Powder is a standard reagent in Southern and Northern blotting procedures. This concentrated powder should be reconstituted in molecular biology grade water to the indicated final volume before use (final solution is a 20x). The 20x concentrate can be used straight or diluted to make SSC working solutions with concentrations as low as 0.5x.

Zastosowanie

Saline-sodium citrate (SSC) Buffer has been used as a washing buffer in microarray technique. It has also been used as a constituent of fluorescence in situ hybridization (FISH) buffer.
Suitable for:
  • Preparing nucleic acids for hybridization
  • Hybridization buffer for Northern and Southern blots
  • Washing buffer for Northern and Southern blots

Cechy i korzyści

  • Multi-purpose use in hybridizations
  • Easily reconstituted and diluted to any concentration needed

Komponenty

Final SSC Buffer 20x Concentrate contains 0.3 M sodium citrate in 3M NaCl.
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produkt powiązany

Numer produktu
Opis
Cennik

Kod klasy składowania

13 - Non Combustible Solids

Klasa zagrożenia wodnego (WGK)

WGK 1

Temperatura zapłonu (°F)

Not applicable

Temperatura zapłonu (°C)

Not applicable

Wybierz jedną z najnowszych wersji:

Certyfikaty analizy (CoA)

Lot/Batch Number
SLBZ0640
SLBS5496
SLBM3250V
SLBD9779V
SLBD9779

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Protokoły

Protokoły Northern i Southern Blot

Poznaj podstawy blottingu Northern i Southern, wraz z protokołami i aplikacjami do przenoszenia makrocząsteczek na nośniki membranowe.

Northern and Southern Blot Protocols & Introduction

An introduction to both Northern and Southern blotting, popular methods for the transfer of macromolecules to membranous support. This article also offers a Southern blot protocol and a northern blot protocol.

Nasz zespół naukowców ma doświadczenie we wszystkich obszarach badań, w tym w naukach przyrodniczych, materiałoznawstwie, syntezie chemicznej, chromatografii, analityce i wielu innych dziedzinach.

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