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Merck

D3287

Sigma-Aldrich

Deoxyribonucleic acid, single stranded from human placenta

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About This Item

Numer CAS:
Numer MDL:
Kod UNSPSC:
12352200

klasa czystości

for molecular biology

opis

For hybridization

Postać

solution

masa cząsteczkowa

(Fragments from 587-831 bp.)

rozpuszczalność

water: 9-12 mg/mL

temp. przechowywania

−20°C

InChI

1S/C15H31N3O13P2/c16-13-1-7(20)11(28-13)5-25-32(21,22)31-9-3-15(18)29-12(9)6-26-33(23,24)30-8-2-14(17)27-10(8)4-19/h7-15,19-20H,1-6,16-18H2,(H,21,22)(H,23,24)

Klucz InChI

AWBASQCACWFTGD-UHFFFAOYSA-N

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Opis ogólny

Human placental DNA is isolated from donor placenta, but will contain some maternal DNA. The DNA fragments are sonicated to produce fragments of consistent size.

Zastosowanie

Sonicated Deoxyribonucleic acid, single stranded from human placenta, was used as blocking agent in Southern hybridization of DNA from human papillomavirus (HPV) positive SiHa, HeLa and CaSki cell-lines. It was used as standard in GC/MS analysis of exocyclic DNA adducts.
Many factors contribute to the signal-to-noise ratio in nucleic acid hybridizations. These factors include the presence of solvent (formamide), hybridization temperature, length of hybridization, volume of hybridization solution, degree and method of agitation, use of blocking reagents, concentration and specific activity of the probe, use of molecular agents to increase the rate of nucleic acid reassociation, and the degree of stringency used during the washing of the membrane.

In order to decrease any non-specific hybridization of the probe to a substrate, blocking agents must be used. Generally, a combination of blocking reagent, detergent, and denatured, fragmented DNA is used to accomplish this. Sigma offers sonicated, denatured DNA from a variety of species for use as a blocking agent in Northern and Southern blotting and other nucleic acid hybridization techniques.

Cechy i korzyści

• High quality human DNA.
• DNA fragments of defined sizes.

Komponenty

DNA is supplied in a solution of 100mM phosphate buffer.  This is a ready to use concentrated solution
of 9-12 mg/ml DNA in 100mM phosphate buffer. However, it will reanneal on standing at room temperature so it is recommended to boil the solution for 10 minutes and then cool on ice for at least 5 minutes prior to use. Cooling on ice will
reduce the chances for reannealing, as it is more likely to reanneal if cooled at room temperature.
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produkt powiązany

Numer produktu
Opis
Cennik

Kod klasy składowania

11 - Combustible Solids

Klasa zagrożenia wodnego (WGK)

WGK 3

Temperatura zapłonu (°F)

Not applicable

Temperatura zapłonu (°C)

Not applicable

Środki ochrony indywidualnej

Eyeshields, Gloves, type N95 (US)


Certyfikaty analizy (CoA)

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Masz już ten produkt?

Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.

Odwiedź Bibliotekę dokumentów

H J Chen et al.
Chemical research in toxicology, 12(12), 1119-1126 (1999-12-22)
Exocyclic DNA adducts have been reported to derive from various exogenous as well as endogenous sources, such as lipid peroxidation. Among them, 1,N(6)-ethenoadenine (epsilonAde) has previously been detected in tissue DNA of untreated rodents and humans by an immunoaffinity/(32)P-postlabeling method.
Mark F Evans et al.
BMC clinical pathology, 3(1), 2-2 (2003-06-13)
BACKGROUND: Over the past five years in situ hybridization techniques employing tyramide amplification reagents have been developed and promise the potential detection of low/single-copy nucleic acid sequences. However the increased sensitivity that tyramide amplification brings about may also lead to
M Iu Mazina et al.
Tsitologiia, 55(4), 218-224 (2013-07-24)
DNA replication begins from multiple sites distributed thoughout the genome and named replication origins. Despite the increasing amount of data on the properties of replication origins, it is still unknown what factors(s) is the primary determinant of ORC localization. Su(Hw)
Non-invasive prenatal aneuploidy testing: technologies and clinical implication.
Brynn Levy et al.
MLO: medical laboratory observer, 45(6), 8-8 (2013-07-24)
Christine Keyser et al.
Medecine sciences : M/S, 29(6-7), 637-641 (2013-07-19)
The authors highlight the opportunities to reconstruct the human Eurasian steppe migration movements with the analyses of nuclear DNA markers (short tandem repeats on autosomal DNA and on the Y chromosome) as well as mitochondrial DNA markers. They studied 26 ancient

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