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Merck

B6755

Sigma-Aldrich

BIS-TRIS propane

≥99.0% (titration)

Synonim(y):

1,3-Bis[tris(hydroxymethyl)methylamino]propane, 2,2′-(Propane-1,3-diylbis(azanediyl))bis(2-(hydroxymethyl)propane-1,3-diol)

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About This Item

Wzór liniowy:
CH2[CH2NHC(CH2OH)3]2
Numer CAS:
Masa cząsteczkowa:
282.33
Beilstein:
1786109
Numer WE:
Numer MDL:
Kod UNSPSC:
12161700
Identyfikator substancji w PubChem:
NACRES:
NA.25

Poziom jakości

Próba

≥99.0% (titration)

Postać

powder

warunki przechowywania

dry at room temperature

metody

HPLC: suitable

kolor

white

przydatny zakres pH

6.3-9.5

pKa (25°C)

(1) 6.8, (2) 9.0

mp

164-165 °C (lit.)

rozpuszczalność

water: 30 % (w/w), clear, colorless

gęstość

1.179 g/cm3 at 20—25 °C
1.189 g/cm3 at 20—25 °C

przydatność

suitable for chromatography
suitable for electrophoresis buffers

Zastosowanie

diagnostic assay manufacturing
general analytical
life science and biopharma

temp. przechowywania

room temp

ciąg SMILES

OCC(CO)(CO)NCCCNC(CO)(CO)CO

InChI

1S/C11H26N2O6/c14-4-10(5-15,6-16)12-2-1-3-13-11(7-17,8-18)9-19/h12-19H,1-9H2

Klucz InChI

HHKZCCWKTZRCCL-UHFFFAOYSA-N

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Opis ogólny

Bis-Tris Propane buffer, also known as BTP, is a zwitterionic biochemical buffer utilized for pH regulation in various laboratory applications, particularly in molecular biology, biochemistry, and electrophoresis. Its wide buffering range, spanning from pH 6.3 to 9.5, is attributed to its two pKa values of 6.8 (pKa1) and 9.0 (pKa2), which are close. This wide range makes it highly versatile for maintaining stable pH conditions in a variety of experiments.

Furthermore, Bis-Tris Propane is a water-soluble buffer substance that plays a crucial role in enhancing the stability and activity of restriction enzymes. Specifically, its buffering capacity is advantageous for experiments that require pH levels as low as 6-7. When compared to Tris buffer, which has a poor buffering capacity below pH 7.5 and exhibits significant pKa fluctuations with temperature changes, Bis-Tris Propane proves to be a superior choice. Additionally, it finds application in conjunction with hydrochloric acid buffer to stabilize farnesyl diphosphate, particularly when isolated from Saccharomyces cerevisiae strains. Notably, it can also serve as a ligand, forming dinuclear hydroxo complexes with Lanthanides(III).

Zastosowanie

BIS-TRIS Propane may be used in the following studies:
  • as a buffer to investigate the influence of anions with varying nucleophilic properties on the autoxidation of oxymyoglobin (MbO2).
  • for purification of glucose-binding protein from membranes of Sulfolobus solfataricus.
  • as a buffer during GeO2 mineralisation.
  • as a buffer component in a study pertaining to environmental research
  • as a buffer component to maintain optimal pH conditions during the purification and preparation of engineered enzymes

Cechy i korzyści

  • Suitable for Biological and Biochemical Research
  • Tested to confirm low levels of heavy metal contamination, ensuring suitability for various applications
  • Effective Buffering from pH 6.3-9.5 (25 °C) with a pKa of 6.8 and 9.0 (25 °C)
  • Highly soluble in water

Inne uwagi

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Kod klasy składowania

11 - Combustible Solids

Klasa zagrożenia wodnego (WGK)

WGK 3

Temperatura zapłonu (°F)

Not applicable

Temperatura zapłonu (°C)

Not applicable

Środki ochrony indywidualnej

Eyeshields, Gloves, type N95 (US)


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Y Satoh et al.
The Journal of biological chemistry, 256(20), 10272-10275 (1981-10-25)
In the presence of a salt in an excess amount, the oxidation of native MbO2 was enhanced considerably above the normal autoxidation in buffr alone with the formation of the corresponding MetMb . anion complex. This anion-induced oxidation of MbO2
Estefania Boix et al.
Dalton transactions (Cambridge, England : 2003), 43(44), 16902-16910 (2014-10-11)
We demonstrate the use of silk based proteins to control the particle/crystallite size during GeO2 formation, using a bio-mimetic approach at circumneutral pH and ambient temperature. Multicrystalline GeO2 was prepared from germanium tetraethoxide (TEOG) in the presence of different silk-based
S V Albers et al.
Journal of bacteriology, 181(14), 4285-4291 (1999-07-10)
The archaeon Sulfolobus solfataricus grows optimally at 80 degrees C and pH 2.5 to 3.5 on carbon sources such as yeast extracts, tryptone, and various sugars. Cells rapidly accumulate glucose. This transport activity involves a membrane-bound glucose-binding protein that interacts
Joseph S Brunzelle et al.
Archives of biochemistry and biophysics, 474(1), 157-166 (2008-04-01)
The three-dimensional structure of the catalytically efficient beta-xylosidase from Selenomonas ruminantium in complex with competitive inhibitor 1,3-bis[tris(hydroxymethyl)methylamino]propane (BTP) was determined by using X-ray crystallography (1.3A resolution). Most H bonds between inhibitor and protein occur within subsite -1, including one between
Sami J O Varjo et al.
Journal of chromatography. A, 1081(1), 92-98 (2005-07-15)
Greater stability of liposome coatings and improved resolution of model steroids in capillary electrochromatography (CEC) were sought by adding small diamines (ethylenediamine, diaminopropane, bis-tris-propane, or N-(2-hydroxyethyl)piperazine-N'-(2-ethanesulfonic acid, HEPES)) to the liposome solution before coating of fused silica capillaries. The phospholipid

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