MOR/0.4R

96042334, human lung, Not specified

• Kod UNSPSC: 41106514

Właściwości

• product name: MOR/0.4R, 96042334
• pochodzenie biologiczne: human lung
• tryb wzrostu: Semi-adherent aggregates
• kariotyp: Not specified
• morfologia: Not specified
• produkty: Not specified
• receptory: Not specified
• metody: cell culture | mammalian: suitable
• powiązane choroby: cancer
• Warunki transportu: dry ice
• temp. przechowywania: −196°C

Opis

Pochodzenie linii komórkowej

Human lung adenocarcinoma, drug-resistant

Opis linii komórkowej

MOR/0.4R has been developed from the parent line, MOR, by continuous exposure to increasing concentrations of doxorubicin (also known as adriamycin). This drug-resistant cell line accumulates lower levels of doxorubicin than the parent line and have been shown to overexpress multi drug resistance-associated protein (MRP). The expression of a 190kDa membrane protein associated with the degree of drug resistance has been indicated. Cells grow as easily detaching aggregates

Zastosowanie

Analysis of acquired drug resistance, MRP studies

Profil DNA

STR-PCR Data: Amelogenin: X
CSF1PO: 10
D13S317: 12
D16S539: 9
D5S818: 12
D7S820: 9
THO1: 7
TPOX: 11
vWA: 15

pożywka hodowlana

RPMI 1640 + 2mM Glutamine + 0.4μg/ml Doxorubicin + 10% Foetal Bovine Serum (FBS).

Rutyna subkultury

Split subconfluent cultures (70-80%) 1:2 to 1:4 seeding at 2-4 x 10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C. It is recommended to culture the cells without drug after resuscitation until the first passage. These cells produce a

Inne uwagi

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