KYSE-70

94072012, human esophagus, Epithelial

• Synonim(y): KY70 Cells, KYSE70 Cells
• Kod UNSPSC: 41106514

Właściwości

• product name: KYSE-70, 94072012
• pochodzenie biologiczne: human esophagus
• tryb wzrostu: Adherent
• kariotyp: Hypotriploid with 8% polyploidy
• morfologia: Epithelial
• produkty: Not specified
• receptory: Not specified
• metody: cell culture | mammalian: suitable
• powiązane choroby: cancer
• Warunki transportu: dry ice
• temp. przechowywania: −196°C

Opis

Pochodzenie linii komórkowej

Human oesophageal squamous cell carcinoma

Opis linii komórkowej

Established from a poorly differentiated invasive oesophageal squamous cell carcinoma resected from middle intra-thoracic oesophagus of a 77-year-old Japanese man prior to treatment. This cell line has been mycoplasma eradicated at ECACC. By short tandem repeat (STR)-PCR analysis the Y chromosome could not be detected in this cell line. It is a known phenomenon that SSC cell lines can lose their Y chromosome.

pożywka hodowlana

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS)

Rutyna subkultury

Split confluent cultures 1:5 to 1:10 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C. Doubling time ~35 hours.

Inne uwagi

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