KYSE-30

94072011, human esophagus, Polygonal

• Synonim(y): KYSE30 Cells
• Kod UNSPSC: 41106514

Właściwości

• Nazwa produktu: KYSE-30, 94072011
• pochodzenie biologiczne: human esophagus
• tryb wzrostu: Adherent
• kariotyp: Aneuploid
• morfologia: Polygonal
• produkty: Not specified
• receptory: Not specified
• metody: cell culture | mammalian: suitable
• powiązane choroby: cancer
• Warunki transportu: dry ice
• temp. przechowywania: −196°C

Opis

Pochodzenie linii komórkowej

Human Asian squamous cell carcinoma

Opis linii komórkowej

KYSE-30 was established from the oesophageal cancer of an untreated 64 year old male. The tumour sample was taken from the mucosal surface of a well differentiated squamous cell carcinoma. The cell line KYSE-30 was established with the use of tumours initially transplanted to athymic mice. The cells are reported to have a doubling time of 20.8 hrs in the exponential growth phase. A p53 mutation at the splice acceptor site of intron 6 and a 12 fold amplification of c-erb B has been reported. KYSE-30 cells express a large number of epidermal growth factor receptors, 1.2x10,000,000 sites/cell.

Zastosowanie

Study of human oesophageal cancer.

pożywka hodowlana

RPMI 1640 + Ham′s F12 (1:1) + 2mM Glutamine + 2% Foetal Bovine Serum (FBS).

Rutyna subkultury

Split sub-confluent cultures (70-80%) 1:10 i.e. seeding at 1x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Inne uwagi

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