KYSE-410

94072023, human esophagus, Epitheloid

• Synonim(y): KYSE0410 Cells, KYSE410 Cells
• Kod UNSPSC: 41106514

Właściwości

• Nazwa produktu: KYSE-410, 94072023
• pochodzenie biologiczne: human esophagus
• tryb wzrostu: Adherent
• kariotyp: Hypotetraploid with 4% polyploidy
• morfologia: Epitheloid
• produkty: Not specified
• receptory: Not specified
• metody: cell culture | mammalian: suitable
• powiązane choroby: cancer
• Warunki transportu: dry ice
• temp. przechowywania: −196°C

Opis

Pochodzenie linii komórkowej

Human oesophageal squamous cell carcinoma

Opis linii komórkowej

Established from the poorly differentiated invasive oesophageal squamous cell carcinoma resected from the cervical oesophagus of a 51-year old Japanese man prior to treatment. Reported to overexpress heparin binding growth factor (hst-1) and cyclin D. This cell line has been mycoplasma eradicated at ECACC. By short tandem repeat (STR)-PCR analysis the Y chromosome could not be detected in this cell line. It is a known phenomenon that SSC cell lines can lose their Y chromosome.

Profil DNA

STR-PCR Data: Amelogenin: X
CSF1PO: 12
D13S317: 11
D16S539: 10,12
D5S818: 13
D7S820: 12
THO1: 8
TPOX: 8,11
vWA: 16,18

pożywka hodowlana

RPMI 1640 + 2mM Glutamine + 5-10% Foetal Bovine Serum (FBS)

Rutyna subkultury

Split confluent cultures 1:5 to 1:10 twice a week i.e. seeding at 2-4x10,000 cells/cm² using trypsin/EDTA;5% CO₂; 37°C. Doubling time ~45h

Inne uwagi

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

Ta strona może zawierać tekst przetłumaczony maszynowo.