DBTRG.05MG

93061119, human brain, Glial

• Kod UNSPSC: 41106514

Właściwości

• product name: DBTRG.05MG, 93061119
• pochodzenie biologiczne: human brain
• tryb wzrostu: Adherent
• kariotyp: 2n = 87-91, hypotetraploid
• morfologia: Glial
• produkty: Not specified
• receptory: Epidermal growth factor (EGF) R
• metody: cell culture | mammalian: suitable
• powiązane choroby: cancer
• Warunki transportu: dry ice
• temp. przechowywania: −196°C

Opis

Pochodzenie linii komórkowej

Human glioblastoma

Opis linii komórkowej

Derived from an adult female with glioblastoma multiforme treated with local brain irradiation and multi drug chemotherapy. By flow cytometry these cells were almost 100% positive for MHC Class I.

Zastosowanie

Tumourigenicity studies

Profil DNA

STR-PCR Data: Amelogenin: X
CSF1PO: 10,11
D13S317: 9
D16S539: 12
D5S818: 12,13
D7S820: 11
THO1: 8
TPOX: 8
vWA: 15,16

pożywka hodowlana

RPMI 1640 + 2mM Glutamine + 1% HT + 1mM Sodium Pyruvate (NaP) + 10% Foetal Bovine Serum (FBS).

Rutyna subkultury

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO₂; 37°C.

Inne uwagi

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