COS-1 Cell Line

88031701, from African green monkey kidney, SV40 transformed, Convention on the International Trade in Endangered Species of Wild Fauna and Flora (CITES)

• Kod UNSPSC: 41106514

Właściwości

• pochodzenie biologiczne: African green monkey kidney
• tryb wzrostu: Adherent
• kariotyp: Not specified
• morfologia: Fibroblast
• produkty: Not specified
• receptory: Not specified
• metody: cell culture | mammalian: suitable
• Warunki transportu: dry ice
• temp. przechowywania: −196°C

Opis

Pochodzenie linii komórkowej

Monkey African green kidney, SV40 transformed

Opis linii komórkowej

Derived from CV-1 simian cells transformed by origin-defective mutant of SV40 which codes for wildtype T Ag. Possible host for propagation of pure populations of recombinant SV40 virus. The cells should be handled under laboratory containment level 2.

Zastosowanie

COS-1 cell line has been used to test the toxicity of various concentrations of iron oxide nanoparticles (for use in medical diagnostics). It has also been used to study the interaction of nanoparticles with their cellular targets.

Virus replication studies: SV40

pożywka hodowlana

DMEM (D6546) + 2 mM L-Glutamine (G7513) + 10% FBS / FCS (F2442).

Rutyna subkultury

Split semi-confluent cultures 1:3 to 1:6, i.e., seeding at 2-4x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37 °C. In order to prevent syncitia formation, do not allow cells become confluent.

Inne uwagi

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