VERO Cell Line

from African green monkey kidney, 84113001, A fibroblast-like kidney cell, non-WHO origin, Convention on International Trade in Endangered Species of Wild Fauna and Flora (CITES)

• Synonim(y): Vero 81 Cells, VeroCCL81 Cells
• Kod UNSPSC: 41106514

Właściwości

• pochodzenie biologiczne: African green monkey kidney
• tryb wzrostu: Adherent
• kariotyp: 2n = 60, modal no. 58
• morfologia: Fibroblast-like
• produkty: Not specified
• receptory: Not specified
• metody: cell culture | mammalian: suitable
• Warunki transportu: dry ice
• temp. przechowywania: −196°C

Opis

Opis ogólny

Vero cells are anchorage-dependent cells and have wide applications in molecular and cell biology research. Vero cells have a pseudo-diploid karyotype and are preferred cell lines for the propagation of various viruses, including poliovirus, rotavirus influenza and rabies virus. These cells are useful hosts for vaccine production.

Pochodzenie linii komórkowej

Monkey African Green kidney

Opis linii komórkowej

Established from the kidney of a normal adult African Green monkey. Susceptible to a wide range of viruses including polio, rubella, arboviruses and reoviruses.

Zastosowanie

VERO cell lines have been used:

• in the propagation of Chandipura virus (CHPV)
• in the cytotoxicity assay to evaluate the antioxidant and anti-viral activities of Capsicum annuum methanolic extracts
• for the propagation of T.cruzi

Virus studies: polio, rubella, arboviruses and reoviruses

pożywka hodowlana

(D6546) + 2 mM (G7513) + 10% (F2442).

Rutyna subkultury

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 1-3x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37 °C.

Inne uwagi

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