MRC-5 PD 19 Cell Line human

5072101, human lung, Fibroblast-like

• Synonim(y): MRC-V Cells, MRC5 Cells, Medical Research Council Cell Strain-5
• Kod UNSPSC: 41106514

Właściwości

• Nazwa produktu: MRC-5 PD 19 Cell Line human, 05072101
• pochodzenie biologiczne: human lung
• opis: Human fetal lung
• tryb wzrostu: Adherent
• kariotyp: 2n=46, diploid
• morfologia: Fibroblast-like
• produkty: Not specified
• receptory: Not specified
• metody: cell culture | mammalian: suitable
• Warunki transportu: dry ice
• temp. przechowywania: −196°C

Opis

Pochodzenie linii komórkowej

Human foetal lung

Opis linii komórkowej

Established from normal lung tissue of a 14 week old male foetus. The cells undergo between 60-70 population doublings before senescence. The virus susceptibility of this line to human viruses is similar to WI-38.

Zastosowanie

It has been used as a cell substrate to study the production of recombinant MVA (modified vaccinia virus Ankara).

Profil DNA

STR-PCR Data: Amelogenin: X,Y
CSF1PO: 11,12
D13S317: 11,14
D16S539: 9,11
D5S818: 11,12
D7S820: 10,11
THO1: 8
TPOX: 8
vWA: 15

pożywka hodowlana

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum FBS / FCS.

Rutyna subkultury

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% Trypsin or Trypsin/EDTA; 5% CO₂; 37°C.

Inne uwagi

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