T9285

Tris-EDTA buffer solution

100X concentrate for Northern and Southern blotting

• Synonim(y): Tris-EDTA wash buffer, TE buffer solution
• Numer MDL: MFCD00236359
• Kod UNSPSC: 41105319
• Identyfikator substancji w PubChem: 329826904
• NACRES: NA.25

Właściwości

• klasa czystości: for molecular biology
• Poziom jakości: 200
• sterylność: 0.2 μm filtered
• Postać: liquid
• pH: 7.8-8.2 (1 × in water)
• Zastosowanie: sample preparation
• obecność zanieczyszczeń: DNase, RNase, none detected
• temp. przechowywania: room temp
• ciąg SMILES: NC(CO)(CO)CO.OC(=O)CN(CCN(CC(O)=O)CC(O)=O)CC(O)=O
• InChI: 1S/C10H16N2O8.C4H11NO3/c13-7(14)3-11(4-8(15)16)1-2-12(5-9(17)18)6-10(19)20;5-4(1-6,2-7)3-8/h1-6H2,(H,13,14)(H,15,16)(H,17,18)(H,19,20);6-8H,1-3,5H2
• Klucz InChI: VLEIUWBSEKKKFX-UHFFFAOYSA-N

Opis

Opis ogólny

Tris EDTA buffer is commonly used in molecular biology for reconstitution and storage of many precipitated biomolecules, including DNA and RNA. Tris-HCl is a commonly used buffer and EDTA is a metal chelating agent. TE buffer solubilizes nucleic acids while protecting the molecules from degradation. It is used in nucleic acid isolation, which may be done prior to nucleic acid gel electrophoresis and Northern or Southern blot hybridization.

Zastosowanie

TE buffer has been used for:

• isolate, resuspend, or store DNA and RNA for PCR or qPCR
• gel electrophoresis, including Northern and Southern blotting
• bacterial cells, for AmpC disk test, to measure β-lactamases released from the cells
• antigen retrieval for immunohistochemistry

Komponenty

TE Buffer contains 1M Tris-HCl (pH approximately 8.0), containing 0.1M EDTA.

Inne uwagi

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Informacja o środkach bezpieczeństwa

• Kod klasy składowania: 12 - Non Combustible Liquids
• Klasa zagrożenia wodnego (WGK): nwg
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable