93283

Tris-EDTA buffer solution

BioUltra, for molecular biology, pH 8.0

• Synonim(y): TE buffer, TE buffer solution
• Numer MDL: MFCD00236359
• Kod UNSPSC: 41105319
• eCl@ss: 32129211
• Identyfikator substancji w PubChem: 329770217
• NACRES: NA.25

Właściwości

• klasa czystości: for molecular biology
• Poziom jakości: 200
• linia produktu: BioUltra
• Postać: liquid
• zanieczyszczenia: DNases, none detected; RNases, none detected; insoluble matter, passes filter test; phosphatases, none detected; proteases, none detected
• pH: 8.0±0.2 (25 °C)
• ślady kationów: Al: ≤1 mg/kg; Ba: ≤1 mg/kg; Bi: ≤1 mg/kg; Ca: ≤5 mg/kg; Cd: ≤1 mg/kg; Co: ≤1 mg/kg; Cr: ≤1 mg/kg; Cu: ≤1 mg/kg; Fe: ≤1 mg/kg; K: ≤20 mg/kg; Li: ≤1 mg/kg; Mg: ≤1 mg/kg; Mn: ≤1 mg/kg; Mo: ≤1 mg/kg; Ni: ≤1 mg/kg; Pb: ≤1 mg/kg; Sr: ≤1 mg/kg; Zn: ≤1 mg/kg
• λ: neat
• absorpcja UV: λ: 260 nm A_max: 0.04; λ: 280 nm A_max: 0.01
• Zastosowanie: sample preparation
• ciąg SMILES: NC(CO)(CO)CO.OC(=O)CN(CCN(CC(O)=O)CC(O)=O)CC(O)=O
• InChI: 1S/C10H16N2O8.C4H11NO3/c13-7(14)3-11(4-8(15)16)1-2-12(5-9(17)18)6-10(19)20;5-4(1-6,2-7)3-8/h1-6H2,(H,13,14)(H,15,16)(H,17,18)(H,19,20);6-8H,1-3,5H2
• Klucz InChI: VLEIUWBSEKKKFX-UHFFFAOYSA-N

Opis

Opis ogólny

Tris EDTA is a biological buffer in research. It is a combination of Tris base and EDTA, a metal chelator. Tris provides buffering capacity, maintaining a stable pH in biological samples, while EDTA helps prevent metal ion interference by chelating divalent metal ions. Tris EDTA is often used in molecular biology techniques, such as DNA and RNA sample preparation, electrophoresis, and enzymatic reactions. It helps maintain the stability and integrity of nucleic acids by inhibiting the activity of nucleases and metal-dependent enzymes.

Zastosowanie

TE buffer is useful for general extraction, suspension, washing, and storage buffer and in the preparation of DNA or RNA. Commonly used for DNA sequencing, restriction enzyme digestion; ligation and polymerase chain reaction (PCR) applications.

Cechy i korzyści

• BioUltra grade - suitable for many molecular biology applications
• DNase, RNase, phosphatase, and protease-free
• High purity and free of trace metals

Inne uwagi

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Informacja o środkach bezpieczeństwa

• Kod klasy składowania: 12 - Non Combustible Liquids
• Klasa zagrożenia wodnego (WGK): nwg
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable
• Środki ochrony indywidualnej: Eyeshields, Gloves