94310

Uricase from Bacillus fastidiosus

lyophilized, ~9 U/mg

• Synonim(y): Urate oxidase
• Numer CAS: 9002-12-4
• Numer EC enzymu: 1.7.3.3 (BRENDA, IUBMB)
• Numer MDL: MFCD00082126
• Kod UNSPSC: 12352204
• Identyfikator substancji w PubChem: 57653596
• NACRES: NA.54

Właściwości

• pochodzenie biologiczne: Bacillus sp. (B. fastidiosus)
• Postać: powder
• jakość: lyophilized
• aktywność właściwa: ~9 U/mg
• warunki przechowywania: dry at room temperature
• stężenie: 90—100%
• kolor: white to faint beige
• rozpuszczalność: 5 mg/mL, clear, colorless to very faintly yellow (in enzyme dilution buffer)
• Zastosowanie: detection
• temp. przechowywania: −20°C
• ciąg SMILES: N.OCC1OC(C(O)C1OP(O)(=O)OCC2OC(C(O)C2O)N3C=CC(=O)NC3=O)N4C=CC(=O)NC4=O
• InChI: 1S/C18H23N4O14P.H3N/c23-5-7-14(13(28)16(34-7)22-4-2-10(25)20-18(22)30)36-37(31,32)33-6-8-11(26)12(27)15(35-8)21-3-1-9(24)19-17(21)29;/h1-4,7-8,11-16,23,26-28H,5-6H2,(H,31,32)(H,19,24,29)(H,20,25,30);1H3
• Klucz InChI: VPXDHZMLJOJKOX-UHFFFAOYSA-N

Opis

Opis ogólny

Research area: Cell signaling

Uricase is present in many organisms, such as, archaea, bacteria, and eukaryotes. The Gut microbiome is the major source of uricase, a homotetrameric enzyme containing four identical 34 kDa subunits.

Zastosowanie

Uricase may be used for the determination of uric acid in biological fluids. It may be helpful in the spectrophotometric determination of purines and purine nucleosides.

Działania biochem./fizjol.

Uricase catalyzes the oxidation of uric acid to 5-hydroxyisourate, which subsequently forms (S)-allantoin. It is involved in purine metabolism, urate degradation and (S)-allantoin formation The absence of functional uricase in humans has significant physiological and pathophysiological implications. It has been associated with higher serum uric acid levels, leading to an increased risk of conditions related to uric acid crystallization, such as gout. Uricase has emerged as an important therapeutic target for the treatment of conditions related to hyperuricemia, such as gout.

Definicja jednostki

1 U corresponds to the amount of enzyme which oxidizes 1 μmole uric acid at pH 9.1 and 37 °C.

Inne uwagi

For the determination of uric acid in biological fluids; In the spectrophotometric determination of purines and purine nucleosides.

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Informacja o środkach bezpieczeństwa

• Piktogramy: GHS08
• Hasło ostrzegawcze: Danger
• Zwroty wskazujące rodzaj zagrożenia: H319,H334,H360FD
• Zwroty wskazujące środki ostrożności: P202 - P261 - P264 - P280 - P305 + P351 + P338 - P308 + P313
• Klasyfikacja zagrożeń: Eye Irrit. 2 - Repr. 1B - Resp. Sens. 1
• Kod klasy składowania: 6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
• Klasa zagrożenia wodnego (WGK): WGK 3
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable
• Środki ochrony indywidualnej: Eyeshields, Gloves, type N95 (US)