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Kluczowe dokumenty
U0880
Uricase from Candida sp.
recombinant, expressed in E. coli, lyophilized powder, ≥2 units/mg solid
Synonim(y):
Urate: oxygen oxoreductase
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About This Item
Polecane produkty
rekombinowane
expressed in E. coli
Poziom jakości
Formularz
lyophilized powder
aktywność właściwa
≥2 units/mg solid
masa cząsteczkowa
35 kDa by SDS-PAGE
temp. przechowywania
−20°C
ciąg SMILES
[P](=O)([O-])(OC3C(OC(C3O)N4C=CC(=O)NC4=O)CO)OCC1OC(C(C1O)O)N2C=CC(=O)NC2=O.[N+H4]
InChI
1S/C18H23N4O14P.H3N/c23-5-7-14(13(28)16(34-7)22-4-2-10(25)20-18(22)30)36-37(31,32)33-6-8-11(26)12(27)15(35-8)21-3-1-9(24)19-17(21)29;/h1-4,7-8,11-16,23,26-28H,5-6H2,(H,31,32)(H,19,24,29)(H,20,25,30);1H3
Klucz InChI
VPXDHZMLJOJKOX-UHFFFAOYSA-N
Opis ogólny
Uricase deficient mice develop uric acid nephropathy and diabetes insipidus which can be mitigated with recombinant uricase treatment.
Uricase is a homo-tetrameric protein with its active sites at the head-to-tail monomer-dimer interface.
Zastosowanie
Uricase from Candida sp. has been used:
- to eliminate urate in plasma samples and evaluate the contribution of urate to the total plasma antioxidant capacity
- to intraperitoneally inject the mice to study the role of uric acid in fibrin extracellular traps (ET) formation
- for elimination of uric acid in ferric reducing/antioxidative power (FRAP) assay to measure the total antioxidative capacity of human plasma sample; in uricase-based biosensors
Działania biochem./fizjol.
Uricase participates in purine catabolism. It catalyzes the conversion of highly insoluble uric acid into 5-hydroxyisourate. Accumulation of uric acid causes liver/kidney damage or chronically causes gout. In mice, a mutation in the gene encoding uricase causes a sudden increase in uric acid. Mice, deficient in this gene, exhibit hyperuricemia, hyperuricosuria, and uric acid crystalline obstructive nephropathy.
Definicja jednostki
One unit will oxidize 1.0 μmole of uric acid to allantoin per minute at pH 8.5 at 25 °C.
Postać fizyczna
Powder; contains citrate and sucrose
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Kod klasy składowania
11 - Combustible Solids
Klasa zagrożenia wodnego (WGK)
WGK 3
Temperatura zapłonu (°F)
Not applicable
Temperatura zapłonu (°C)
Not applicable
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