436143

Sodium dodecyl sulfate

ACS reagent, ≥99.0%

• Synonim(y): Dodecyl sodium sulfate, Dodecyl sulfate sodium salt, Lauryl sulfate sodium salt, SDS, Sodium lauryl sulfate
• Wzór liniowy: CH₃(CH₂)₁₁OSO₃Na
• Numer CAS: 151-21-3
• Masa cząsteczkowa: 288.38
• Beilstein: 3599286
• Numer WE: 205-788-1
• Numer MDL: MFCD00036175
• Kod UNSPSC: 12161900
• eCl@ss: 39093306
• Identyfikator substancji w PubChem: 24867284
• NACRES: NA.21

Właściwości

• klasa czystości: ACS reagent
• Poziom jakości: 200
• opis: anionic; 62
• Próba: ≥99.0%
• Formularz: solid
• masa cząsteczkowa: 288.38 g/mol
• zanieczyszczenia: ≤0.06 meq/g Titr. Base; ≥96.0% fatty alcohols; <4.0% unsulfated alcohols
• strata: ≤1.0% loss on drying
• mp: 204-207 °C (lit.)
• rozpuszczalność: H₂O: soluble 1 gm in 10 ml (opalescent solution)
• ślady kationów: heavy metals (as Pb): ≤0.002%
• λ: 3 %H₂O reference
• absorpcja UV: λ: 220-350 nm A_max: ≤0.1
• HLB: 40
• ciąg SMILES: [Na+].CCCCCCCCCCCCOS([O-])(=O)=O
• InChI: 1S/C12H26O4S.Na/c1-2-3-4-5-6-7-8-9-10-11-12-16-17(13,14)15;/h2-12H2,1H3,(H,13,14,15);/q;+1/p-1
• Klucz InChI: DBMJMQXJHONAFJ-UHFFFAOYSA-M

Opis

Opis ogólny

Sodium dodecyl sulfate (SDS), also known as sodium lauryl sulfate, is a versatile anionic surfactant with a broad range of applications in cell biology, biochemistry, and various industries. Its amphiphilic nature, characterized by a hydrophobic hydrocarbon tail and a hydrophilic sulfate head group, makes it an effective detergent capable of solubilizing and denaturing proteins, lipids, and other hydrophobic molecules.

In protein biochemistry, SDS plays a pivotal role in protein extraction and SDS-PAGE (sodium dodecyl sulfate-polyacrylamide gel electrophoresis). Its ability to denature proteins by disrupting their secondary and tertiary structures allows for their uniform migration through a polyacrylamide gel matrix based on their molecular weight. This technique, SDS-PAGE, has become an indispensable tool for analyzing protein size and purity.

Beyond protein analysis, SDS also finds applications in nucleic acid extraction and hybridization techniques. Its detergent properties aid in the lysis of cells and the solubilization of cellular components, facilitating the extraction of DNA and RNA. This makes SDS a valuable reagent in various molecular biology protocols, including PCR (polymerase chain reaction) and Southern blotting. Sodium dodecyl sulfate stands as a versatile and widely used surfactant with a diverse range of applications due to its ability to solubilize, denature, and separate biological molecules for cell biology and biochemical research.

Zastosowanie

Anionic detergent

Sodium dodecyl sulfate was used as a test compound for studying that whether Bacillus amyloliquefaciens KPS46 can induce priming, signaling, protein production as well as systemic protection in soybean (Glycine max L.) against several diseases.

Działania biochem./fizjol.

Sodium dodecyl sulfate (SDS) is an anionic detergent that can form complexes with protein through hydrophobic interactions. Studies have reported that the hydrodynamic functions of protein-SDS complexes are governed by the length of their polypeptide chains. Thus, SDS-based electrophoretic techniques can separate protein molecules based on their molecular weights. Additionally, SDS can solubilize cell membranes and can extract membrane-bound proteins.

Cechy i korzyści

• Highly versatile surfactant for your molecular biology and biochemical research
• Tested to confirm low levels of heavy metal contamination, ensuring suitability for various applications

Inne uwagi

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Informacja o środkach bezpieczeństwa

• Piktogramy: GHS02,GHS05,GHS07
• Hasło ostrzegawcze: Danger
• Zwroty wskazujące rodzaj zagrożenia: H228,H302 + H332,H315,H318,H335,H412
• Zwroty wskazujące środki ostrożności: P210 - P273 - P280 - P301 + P312 - P304 + P340 + P312 - P305 + P351 + P338
• Klasyfikacja zagrożeń: Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3 - Eye Dam. 1 - Flam. Sol. 2 - Skin Irrit. 2 - STOT SE 3
• Organy docelowe: Respiratory system
• Kod klasy składowania: 4.1B - Flammable solid hazardous materials
• Klasa zagrożenia wodnego (WGK): WGK 2
• Temperatura zapłonu (°F): 338.0 °F
• Temperatura zapłonu (°C): 170 °C
• Środki ochrony indywidualnej: Eyeshields, Faceshields, Gloves, type P3 (EN 143) respirator cartridges