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PROTGA-RO
Roche
Protein G Agarose
>98% (HPLC and SDS-PAGE), suspension
Synonim(y):
agarose, protein g
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About This Item
Kod UNSPSC:
41116133
Polecane produkty
Próba
>98% (HPLC and SDS-PAGE)
Formularz
suspension
opakowanie
pkg of 15 mL (05015952001 [settled resin volume])
pkg of 2 mL (11719416001 [settled resin volume])
pkg of 5 mL (11243233001 [settled resin volume])
producent / nazwa handlowa
Roche
temp. przechowywania
2-8°C
Opis ogólny
Protein G was initially isolated from G148, a human group G Streptococcal strain. It is a cell wall protein and shows high affinity to IgG (immunoglobulin G). It has a putative molecular weight of 30,000Da. It interacts with all subclasses of human IgG and also binds to mouse, rabbit and goat IgG.
Specyficzność
Protein G is a cell wall protein, isolated from a specific bacterial strain, which has specific binding sites for certain classes of immunoglobulins from different species. Protein G binds nearly all subclasses of IgG, but no other classes of immunoglobulins.
Binding capacity: 20mg human IgG (polyclonal)/ml (the IgG is loaded at pH 7.0, and eluted with 200mM glycine, pH 2.8)
Structure: recombinant Protein G (E. coli, Mr = 22,000) is covalently coupled to crosslinked 4% agarose beads: 2.5mg Protein G (>98% pure)/1ml gel.
Binding capacity: 20mg human IgG (polyclonal)/ml (the IgG is loaded at pH 7.0, and eluted with 200mM glycine, pH 2.8)
Structure: recombinant Protein G (E. coli, Mr = 22,000) is covalently coupled to crosslinked 4% agarose beads: 2.5mg Protein G (>98% pure)/1ml gel.
Zastosowanie
Affinity chromatography using Protein G Agarose, like Protein A Agarose, is the method of choice for purification of IgG from many species. It has been used as a pre-clearing agent during-
- Ubiquitination assay
- Immunoprecipitation
Cechy i korzyści
Contents
Preswollen gel, ready-to-use, 2ml or 5ml gel (settled gel volume) in buffer that contains 14-19% ethanol as a preservative, nonsterile.
Protein G content: 2.5mg/ml preswollen gel
Preswollen gel, ready-to-use, 2ml or 5ml gel (settled gel volume) in buffer that contains 14-19% ethanol as a preservative, nonsterile.
Protein G content: 2.5mg/ml preswollen gel
Jakość
Purity: >98% (HPLC, SDS-PAGE); entirely free from staphylococcal enterotoxins.
Inne uwagi
For life science research only. Not for use in diagnostic procedures.
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Hasło ostrzegawcze
Warning
Zwroty wskazujące rodzaj zagrożenia
Zwroty wskazujące środki ostrożności
Klasyfikacja zagrożeń
Eye Irrit. 2 - Flam. Liq. 3
Kod klasy składowania
3 - Flammable liquids
Klasa zagrożenia wodnego (WGK)
WGK 1
Temperatura zapłonu (°F)
102.2 °F
Temperatura zapłonu (°C)
39 °C
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Global Trade Item Number
| SKU | GTIN |
|---|---|
| 5015952001 | 4061832422589 |
| 11243233001 | 4061838250896 |
| 11719416001 | 4061838251039 |
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