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Key Documents

16-201

Sigma-Aldrich

Protein G Agarose/Salmon Sperm DNA, 2.5 mL

for use in chromatin immunoprecipitations (ChIP assays)

Synonim(y):

ChIP agarose beads, ChIP agaraose G beads

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About This Item

Kod UNSPSC:
12352202
eCl@ss:
32160405
NACRES:
NA.32

Poziom jakości

Postać

liquid

producent / nazwa handlowa

Upstate®

metody

ChIP: suitable

przydatność

suitable for immunoprecipitation

Warunki transportu

wet ice

Opis ogólny

Recombinant Protein G covalently bound to agarose by cyanogen bromide linkage. Sonicated salmon sperm DNA blocks non-specific DNA binding sites on protein G agarose when collecting immunocomplexes for chromatin immunoprecipitations (ChIP assays).
Recombinant protein G has a very high affinity for the Fc regions of IgG molecules. Immoblisation of protein G on beads creates an affinity resin which can be used to purify IgG fractions from crude serum, ascites fluid or cell cuture media.

Opakowanie

2.5 mL packed beads provided as a 50% gel slurry for a final volume of 5 mL.

Jakość

Routinely evaluated in Chromatin Immunoprecipitation for use to pre-clear the supernatant from sodium butyrate treated HeLa cells and subsequent immunoprecipitation of DNA cross-linked to acetylated histones

Postać fizyczna

2.5 mL packed beads containing 1 mg sonicated salmon sperm DNA, 2.5 mg BSA and approximately 5 mg recombinant Protein G. Provided as a 50% gel slurry for a final volume of 5 mL per vial. Suspended in 10 mM Tris-HCl, 1 mM EDTA, pH 8.0, containing 0.05% sodium azide. Liquid suspension.

Przechowywanie i stabilność

1 year at 4°C from date of shipment.

Informacje prawne

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Oświadczenie o zrzeczeniu się odpowiedzialności

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Kod klasy składowania

10 - Combustible liquids

Klasa zagrożenia wodnego (WGK)

WGK 1


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Masz już ten produkt?

Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.

Odwiedź Bibliotekę dokumentów

MUC1 regulates nuclear localization and function of the epidermal growth factor receptor.
Bitler, BG; Goverdhan, A; Schroeder, JA
Journal of Cell Science null
Kyster K Nanan et al.
Nucleic acids research, 45(22), 12780-12797 (2017-12-16)
Actively transcribed genes adopt a unique chromatin environment with characteristic patterns of enrichment. Within gene bodies, H3K36me3 and cytosine DNA methylation are elevated at exons of spliced genes and have been implicated in the regulation of pre-mRNA splicing. H3K36me3 is
Fen Hu et al.
Experimental and therapeutic medicine, 15(1), 569-575 (2018-02-06)
Human embryo lung cellular protein interacting with severe acute respiratory syndrome-coronavirus nonstructural protein-10 (HEPIS) is a novel transcriptional repressor, the expression profile and promoter activity of which have not been well studied. In the present study, in situ hybridization of
Kyung-Soon Park et al.
Stem cells (Dayton, Ohio), 31(2), 282-292 (2012-11-22)
Self-renewal and pluripotency are hallmark properties of pluripotent stem cells, including embryonic stem cells (ESCs) and iPS cells. Previous studies revealed the ESC-specific core transcription circuitry and showed that these core factors (e.g., Oct3/4, Sox2, and Nanog) regulate not only
Yang Tang et al.
Molecular plant, 10(2), 260-273 (2016-11-24)
Light is a crucial environmental signal that promotes photomorphogenesis, the developmental process with a series of light-dependent alterations for plants to adapt various external challenges. Chromatin modification has been proposed to be involved in such light-mediated growth, but the underlying

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