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P3391

Millipore

Protein A-Sepharose™ from Staphylococcus aureus

lyophilized powder

Synonim(y):

Protein A–Agarose

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About This Item

Numer MDL:
MFCD00165563
Kod UNSPSC:
41106500
NACRES:
NA.56

Postać

lyophilized powder

Poziom jakości

200

opakowanie

glass bottle of 1 g
glass bottle of 1.5 g
glass bottle of 250 mg
glass bottle of 5 g

warunki przechowywania

do not freeze

zakres etykietowania

3 mg per mL

metody

affinity chromatography: suitable

kolor

white to gray

macierz

Sepharose CL-4B

aktywacja macierzy

cyanogen bromide

MAR

amino

spacer macierzy

1 atom

pojemność

≥16 mg/mL binding capacity (human IgG)

rozpuszczalność

water: 1 g/L at 20 °C

przydatność

conforms to structure for swelling capacity (1g swells to 4 to 5 mL)

temp. przechowywania

2-8°C

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Opis ogólny

Protein A-Sepharose when linked with antibody, helps improving antigen binding capacity of IgG crosslinked to column matrices. In this case, binding happens between Protein A and Fc portion of the IgG molecule leaving antigen specific sites free.

Zastosowanie

Protein A-Sepharose was used:
  • for immunoprecipitated placentas for protein and RNA analyses using Western blot technique.
  • in chromatin immunoprecipitation to understand the link between Mediator and remodelling of chromatin structure at specific promoters.
  • as lysis buffer to remove endogenous G-type Igs in protein extraction of brain samples from female rats.
  • in immunoprecipitation of [32P] phosphomyristin C of rabbit antiserum, to study the down-regulation of protein kinase C (PKC) and phosphomyristin C (PMC) during T-cell development.
Protein A-Sepharose™ is used for affinity chromatography, antibody purification and characterization, immunoaffinity matrices, protein chromatography, protein A, G and L resins, and recombinant protein expression and analysis. Protein A-Sepharose™ has been used to develop strategies for investigating protein interactions, to improve the detection of celiac disease, and to study diabetes in children.

Ilość

Swelling: 1 g swells to approx. 4 ml.

Postać fizyczna

Supplied as lyophilized powder stabilized with lactose and dextran.

Informacje prawne

Sepharose is a trademark of Cytiva
This page may contain text that has been machine translated.

produkt powiązany

Numer produktu
Opis
Cennik

Kod klasy składowania

11 - Combustible Solids

Klasa zagrożenia wodnego (WGK)

WGK 3

Temperatura zapłonu (°F)

Not applicable

Temperatura zapłonu (°C)

Not applicable

Środki ochrony indywidualnej

Eyeshields, Gloves, type N95 (US)

Certyfikaty analizy (CoA)

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Masz już ten produkt?

Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.

Odwiedź Bibliotekę dokumentów

Lionel Gissot et al.
Plant physiology, 142(3), 931-944 (2006-10-10)
The sucrose nonfermenting-1 protein kinase (SNF1)/AMP-activated protein kinase subfamily plays a central role in metabolic responses to nutritional and environmental stresses. In yeast (Saccharomyces cerevisiae) and mammals, the beta- and gamma-noncatalytic subunits are implicated in substrate specificity and subcellular localization
P Hornbeck et al.
Molecular and cellular biology, 9(9), 3727-3735 (1989-09-01)
The regulation and expression of protein kinase C (PKC) and phosphomyristin C (PMC) (a principal substrate of PKC which is the major myristylated protein in lymphocyte and glioma lines that express it) in murine B and T lymphocytes were investigated.
M Iezzi et al.
Molecular endocrinology (Baltimore, Md.), 13(2), 202-212 (1999-02-11)
Insulin-secreting cells express four GTPases of the Rab3 family. After separation of extracts of INS-1 cells on a sucrose density gradient, the bulk of the A, B, and C isoforms was recovered in the fractions enriched in insulin-containing secretory granules.
Tracie A Hennen-Bierwagen et al.
Plant physiology, 149(3), 1541-1559 (2009-01-27)
Starch biosynthetic enzymes from maize (Zea mays) and wheat (Triticum aestivum) amyloplasts exist in cell extracts in high molecular weight complexes; however, the nature of those assemblies remains to be defined. This study tested the interdependence of the maize enzymes
Adam F L Hurlstone et al.
The Biochemical journal, 364(Pt 1), 255-264 (2002-05-04)
A highly conserved multisubunit enzymic complex, SWI/SNF, participates in the regulation of eukaryote gene expression through its ability to remodel chromatin. While a single component of SWI/SNF, Swi2 or a related protein, can perform this function in vitro, the other
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Protokoły

Immunoprecipitation Procedure

To determine the molecular weights of protein antigens, to study protein/protein interactions, to determine specific enzymatic activity, to monitor protein post-translational modifications and to determine the presence and quantity of proteins.

Procedura immunoprecypitacji

Techniki określania masy cząsteczkowej antygenu białkowego, interakcji białkowych, aktywności enzymatycznej i modyfikacji potranslacyjnych.

Nasz zespół naukowców ma doświadczenie we wszystkich obszarach badań, w tym w naukach przyrodniczych, materiałoznawstwie, syntezie chemicznej, chromatografii, analityce i wielu innych dziedzinach.

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