IP05
Protein G Plus/Protein A Agarose Suspension
Protein G PLUS/Protein A-Agarose mixture specifically formulated for immunoprecipitation.
Synonim(y):
Affinity resin, Protein G/Agarose
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About This Item
Kod UNSPSC:
41116133
NACRES:
NA.56
Polecane produkty
Postać
slurry (Liquid)
zawiera
≤0.1% sodium azide as preservative
producent / nazwa handlowa
Calbiochem®
warunki przechowywania
do not freeze
metody
protein purification: suitable
przydatność
suitable for microbiology
Warunki transportu
wet ice
temp. przechowywania
2-8°C
Opis ogólny
Designed for immunoprecipitation applications. This product is blocked with BSA to reduce non-specific binding and cannot be used for purification.
Protein G PLUS/Protein A-Agarose mixture specifically formulated for immunoprecipitation.
Zastosowanie
Immunoprecipitation (see comments)
Ostrzeżenie
Toxicity: Standard Handling (A)
Postać fizyczna
33% slurry in PBS.
Inne uwagi
Agarose solution is supplied ready to use. Protein G Plus/Protein A Agarose immunoprecipitation reagent is blocked with BSA and should not be used for immunoglobulin purification or covalent cross-linking. For immunoprecipitation reactions 15 µl of solution per µg primary antibody is recommended. Preclearing will minimize extra bands resulting from nonspecific precipitation. To preclear, add to the sample 20 µl of agarose conjugate and 1 µg of normal IgG from the same species as the immunoprecipitating antibody. When immunoblotting is used for detection, some secondary antibodies can react nonspecifically with BSA or other proteins present at high concentrations in the sample. This can be eliminated by reducing the concentration of secondary antibody.
Informacje prawne
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
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Kod klasy składowania
11 - Combustible Solids
Klasa zagrożenia wodnego (WGK)
WGK 1
Temperatura zapłonu (°F)
Not applicable
Temperatura zapłonu (°C)
Not applicable
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