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ENDOGLUS-RO
Roche
Endoproteinase Glu-C Sequencing Grade
from Staphylococcus aureus V8
Synonim(y):
protease
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About This Item
Polecane produkty
pochodzenie biologiczne
Staphylococcus aureus (V8)
Formularz
lyophilized (salt-free)
lyophilized
aktywność właściwa
≥15 units/mg protein
masa cząsteczkowa
Mr 27 kDa
opakowanie
pkg of 3 × 50 μg (11047817001)
pkg of 50 μg (11420399001)
producent / nazwa handlowa
Roche
optymalne pH
4.0-7.8
temp. przechowywania
2-8°C
Powiązane kategorie
Opis ogólny
Endoproteinase Glu-C Sequencing Grade is a serine protease isolated from Staphylococcus aureus V8 as a highly purified and specific protease. Inhibitors of Endoproteinase Glu-C are DFP, α2-macroglobulin and TLCK.
Specyficzność
Serine protease that very specifically hydrolyzes peptide bonds at the carboxylic side of Glu (in ammonium bicarbonate, pH 7.8, or ammonium acetate buffer, pH 4.0), or Glu and Asp (in phosphate buffer, pH 7.8). The specificity of Endoproteinase Glu-C Sequencing Grade is verified with the oxidized B-chain of insulin (insulin Box) as substrate. High concentrations of Endoproteinase Glu-C Sequencing Grade (1 part by weight enzyme with 10 parts by weight insulin Box) are incubated to detect traces of impurities, for example, contaminating proteases.
Heat inactivation: Most proteases sequencing grade are stopped by boiling for 5 minutes.
Heat inactivation: Most proteases sequencing grade are stopped by boiling for 5 minutes.
Zastosowanie
Use Endoproteinase Glu-C Sequencing Grade for protein structure analysis and for sequence analysis.
Cechy i korzyści
Free of impurities that may interfere with separation of peptides in RP HPLC.
Uwaga dotycząca przygotowania
Working concentration: 1/100 to 1/20 (w/w)
With a minimum amount of 1 to 5 μg insulin Box, sufficient levels can be detected using the Column Aquapore RUP 300, 4.6 x 100 nm, 7 μm.
Working solution: Solvent is recommended in double-distilled water.
Solvents other than water have not been tested; Roche recommends using 50 mM Hepes, pH 7.8.
Storage conditions (working solution): +2°C to +8°C for 1-2 days.
Stability: At +2 to +8°C within specification range for 12 months; stored dry.
With a minimum amount of 1 to 5 μg insulin Box, sufficient levels can be detected using the Column Aquapore RUP 300, 4.6 x 100 nm, 7 μm.
Working solution: Solvent is recommended in double-distilled water.
Solvents other than water have not been tested; Roche recommends using 50 mM Hepes, pH 7.8.
Storage conditions (working solution): +2°C to +8°C for 1-2 days.
Stability: At +2 to +8°C within specification range for 12 months; stored dry.
Rekonstytucja
Lyophilized Endoproteinase Glu-C sequencing grade is reconstituted in double-dist. water. To avoid autolysis, the incubation temperature should not exceed 25 °C.
Inne uwagi
For life science research only. Not for use in diagnostic procedures.
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Hasło ostrzegawcze
Danger
Zwroty wskazujące rodzaj zagrożenia
Zwroty wskazujące środki ostrożności
Klasyfikacja zagrożeń
Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
Organy docelowe
Respiratory system
Kod klasy składowania
11 - Combustible Solids
Klasa zagrożenia wodnego (WGK)
WGK 1
Temperatura zapłonu (°F)
does not flash
Temperatura zapłonu (°C)
does not flash
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