324708

Endoproteinase Asp-N, Excision Grade, Pseudomonas fragi

• Synonim(y): Endoproteinase Asp-N, Excision Grade, Pseudomonas fragi
• Numer CAS: 9001-92-7
• Numer EC enzymu: 3.4.24.33 (BRENDA, IUBMB)
• Kod UNSPSC: 12352202
• NACRES: NA.54

Właściwości

• klasa czystości: Proteomics Grade
• Poziom jakości: 100
• Próba: ≥90% (SDS-PAGE)
• Postać: lyophilized solid
• aktywność właściwa: ≥20,000 units/mg protein
• producent / nazwa handlowa: Calbiochem^®
• warunki przechowywania: OK to freeze
• kolor: white
• rozpuszczalność: water: soluble
• Warunki transportu: ambient
• temp. przechowywania: 2-8°C

Opis

Opis ogólny

Native endoproteinase Asp-N from Pseudomonas fragi. Designed for protein sequencing or sequence verification, analysis of protein structural domains, and cleavage of fusion proteins. Metalloprotease that specifically hydrolyzes proteins and the N-terminal side of aspartic acid and cysteic acid residues. Inhibited by aprotinin, DFP, leupeptin, and TLCK.

Native endoproteinase Asp-N from Pseudomonas fragi. Designed for protein sequencing or sequence verification, analysis of protein structural domains, and cleavage of fusion proteins. Metalloprotease that specifically hydrolyzes proteins at the N-terminal side of aspartic acid and cysteic acid residues. Inhibited by aprotinin, DFP, leupeptin, and TLCK. Suggested working concentration: 1:20 to 1:100 (protease:protein by weight) for sequence analysis.

Ostrzeżenie

Toxicity: Harmful (C)

Definicja jednostki

One unit is defined as the amount of enzyme that will release a sufficient amount of azo dye from AZOCOLL Substrate (Cat. No. 194932) to produce an increase in absorbance at 520 nm of 0.001 per min at 37°C, pH 7.5.

Postać fizyczna

Lyophilized from 10 mM Tris-HCl, pH 7.5.

Rekonstytucja

Following reconstitution, aliquot and freeze (-20°C) for long term storage or refrigerate (4°C) for short term storage. Stock solutions are stable for up to 2 days at 4°C or for up to 1 month at -20°C.

Inne uwagi

Drapeau, G.R. 1980. J. Biol. Chem.255, 839.

Informacje prawne

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

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Informacja o środkach bezpieczeństwa

• Piktogramy: GHS08,GHS07
• Hasło ostrzegawcze: Danger
• Zwroty wskazujące rodzaj zagrożenia: H315,H319,H334,H335
• Zwroty wskazujące środki ostrożności: P261 - P264 - P271 - P302 + P352 - P304 + P340 + P312 - P305 + P351 + P338
• Klasyfikacja zagrożeń: Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
• Organy docelowe: Respiratory system
• Kod klasy składowania: 11 - Combustible Solids
• Klasa zagrożenia wodnego (WGK): WGK 1