ENDOLYSS-RO

Endoproteinase Lys-C Sequencing Grade

from Lysobacter enzymogenes

• Synonim(y): lys-c, lysyl endopeptidase, protease
• Numer EC enzymu: 3.4.21.50 (BRENDA, IUBMB)
• Kod UNSPSC: 12352204

Właściwości

• pochodzenie biologiczne: bacterial (Lysobacter enzymogenes)
• klasa czystości: protein sequencing grade
• Postać: lyophilized
• aktywność właściwa: ≥200 units/mg protein (at 25 °C, with Chromozym PL)
• masa cząsteczkowa: 33 kDa by SDS-PAGE (reducing)
• oczyszczone przez: electrophoresis
• opakowanie: pkg of 3 × 5 μg (11047825001); pkg of 5 μg (11420429001)
• producent / nazwa handlowa: Roche
• warunki przechowywania: (Keep container tightly closed in a dry and well-ventilated place.)
• metody: activity assay: suitable
• kolor: white
• optymalne pH: 8.5-8.8
• zakres pH: 5-12
• rozpuszczalność: water: soluble
• przydatność: suitable for enzyme test
• numer dostępu UniProt: Q7M135
• temp. przechowywania: 2-8°C

Opis

Opis ogólny

Endoproteinase Lys-C Sequencing Grade is isolated from Lysobacter enzymogenes as a highly purified and specific protease. Endoproteinase Lys-C from Lysobacter enzymogenes is a member of the peptidase S1 family. It is mostly used for the initial fragmentation of polypeptide chains in protein sequence analysis.

Specyficzność

This enzyme is a serine protease that specifically hydrolyzes amide, ester, and peptide bonds at the carboxylic side of Lys (in Tris-HCl buffer, pH 7.0 to 9.0).
Heat inactivation: Enzyme can be denatured using heat (5 min at 100 °C) or by TCA.

Zastosowanie

Use Endoproteinase Lys-C Sequencing Grade  for protein structure analysis and for sequence analysis. It is suitable to digest proteins in solution, in polyacrylamide gels or on blotting membranes.

Uwaga dotycząca przygotowania

Storage conditions (working solution): +2 to +8 °C

Rekonstytucja

Lyophilized Endoproteinase Lys-C sequencing grade is reconstituted in 50 μl double-dist. water. This results in a buffer concentration of 50 mM Hepes, pH 8.0, 10 mM EDTA and 5 mg/ml raffinose. To avoid autolysis, the incubation temperature should not exceed 37 °C.
Stable in 4 M urea.

Inne uwagi

For life science research only. Not for use in diagnostic procedures.

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Informacja o środkach bezpieczeństwa

• Kod klasy składowania: 11 - Combustible Solids
• Klasa zagrożenia wodnego (WGK): WGK 2
• Temperatura zapłonu (°F): does not flash
• Temperatura zapłonu (°C): does not flash