NGLYFL-RO

N-Glycosidase F

recombinant form of the gene from Flavobacterium meningosepticum

• Synonim(y): N-Glycosidase F, PNGase F, Peptide-N-glycosidase F, Peptide-N⁴-(acetyl-β-glucosaminyl)-asparagine amidase
• Numer EC enzymu: 3.5.1.52 (BRENDA, IUBMB)
• Kod UNSPSC: 12352204

Właściwości

• pochodzenie biologiczne: bacterial (Flavobacterium meningosepticum)
• Poziom jakości: 100
• rekombinowane: expressed in E. coli
• białko sprzężone: (N-linked)
• Próba: ≥90% (SDS-PAGE)
• Postać: lyophilized
• aktywność właściwa: >25000 units/mg protein
• masa cząsteczkowa: 35.5 kDa
• oczyszczone przez: electrophoresis
• opakowanie: pkg of 100 U (11365185001); pkg of 250 U (11365193001)
• producent / nazwa handlowa: Roche
• warunki przechowywania: (Keep container tightly closed in a dry and well-ventilated place.)
• metody: activity assay: suitable
• kolor: colorless
• optymalne pH: 7.0-8.0
• rozpuszczalność: water: soluble
• przydatność: suitable for enzyme test
• obecność zanieczyszczeń: Endoglycosidase F, none detected; a-Fucosidase, present; b-Galactosidase, present; b-N-Acetylhexosaminidase, present; dA(17h ≤100 units, present
• temp. przechowywania: 2-8°C

Opis

Opis ogólny

N-Glycosidase F (PNGase F) is a potent enzyme which hydrolyzes at glycosylamine linkage. It also helps in generating a carbohydrate-free peptide and oligosaccharide with di-N-acetylchitobiose unit.

N-glycosidase F, also known as PNGase F, is an asparagine amidase enzyme derived from Flavobacterium meningosepticum. It is widely used as a valuable tool in protein research to investigate and analyze N-glycosylation.

Specyficzność

Hydrolyzes all types of N-glycan chains from glycopeptides and glycoproteins unless they carry α1,3-linked core fucose residues present in insect and plant glycoproteins. Free of contaminating proteolytic activities (x = H or sugar[s]) according to current quality control procedures.

Zastosowanie

N-Glycosidase F has been used for deglycosylation of N-glycoproteins.

Use N-glycosidase F to cleave all types of asparagine-bound N-glycans, provided that the amino group as well as the carboxyl group are present in a peptide linkage, and that the oligosaccharide has the minimum length of the chitobiose core unit. The reaction products are ammonia, aspartic acid (in the peptide chain), and the complete oligosaccharide.
Note: N-Glycosidase F, recombinant is also available as a solution.

Definicja jednostki

One unit is the enzyme activity which hydrolyzes 1 nmol dabsyl fibrin glycopeptide or 0.2 nmol dansyl fetuin glycoprotein within 1 minute at 37 °C and pH 7.8.

Postać fizyczna

Clear, colorless solution after reconstitution

Uwaga dotycząca przygotowania

Storage conditions (working solution): 2 to 8 °C
The reconstituted solution is stable at 2 to 8 °C for at least four weeks.

Rekonstytucja

Dissolving the content in 0.1 ml redist water (100 unit package) or 0.25 ml double-dist. water (250 unit package) respectively, results in a concentration of 100 mM sodium phosphate buffer, 25 mM EDTA, pH 7.2.
Note: N-Glycosidase F, recombinant is also available as solution with 50% glycerol.

Inne uwagi

For life science research only. Not for use in diagnostic procedures.

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Informacja o środkach bezpieczeństwa

• Kod klasy składowania: 13 - Non Combustible Solids
• Klasa zagrożenia wodnego (WGK): WGK 2
• Temperatura zapłonu (°F): does not flash
• Temperatura zapłonu (°C): does not flash