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Merck

11685619910

Roche

Mieszanka do znakowania RNA fluoresceiną

solution, suitable for Northern blotting, suitable for Southern blotting, suitable for hybridization

Synonim(y):

Etykietowanie RNA

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About This Item

Kod UNSPSC:
41105500

Formularz

solution

Poziom jakości

zastosowanie

sufficient for 20 reactions

opakowanie

pkg of 40 μL

producent / nazwa handlowa

Roche

metody

Northern blotting: suitable
Southern blotting: suitable
hybridization: suitable

temp. przechowywania

−20°C

Opis ogólny

Fluorescein RNA Labeling Mix is a convenient nucleotide mixture for the labeling of RNA with fluorescein-12-uridine triphosphate (UTP).

Specyficzność

Heat inactivation: Stop the reaction by adding 2μl 0.2M EDTA (pH 8.0).

Zastosowanie

Fluorescein-labeled RNA has been used in a variety of hybridization techniques such as:
  • in situ hybridization to tissues
  • Southern blots
  • northern blots
  • plaque or colony lifts
  • RNase protection experiments

Jakość

The mix is function tested in combination with the DIG RNA Labeling Kit.

Zasada

Fluorescein-labeled, single-stranded RNA probes of defined length are generated by in vitro transcription. Fluorescein-12-UTP is incorporated by SP6, T7, and T3 RNA polymerases at approximately every 20 to 25th nucleotide of the transcript under standard conditions. The Fluorescein RNA Labeling Mix is specifically designed for use with the RNA polymerases SP6, T7, and T3, which are supplied with an optimized transcription buffer.

Uwaga dotycząca przygotowania

10x concentrated NTP labeling mixture: 10mM ATP, 10mM CTP, 10mM GTP, 6.5mM UTP, 3.5mM Fluorescein-12-UTP, pH 7.5 (+20°C).

Inne uwagi

For life science research only. Not for use in diagnostic procedures.
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Kod klasy składowania

12 - Non Combustible Liquids

Klasa zagrożenia wodnego (WGK)

nwg

Temperatura zapłonu (°F)

No data available

Temperatura zapłonu (°C)

No data available


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Produkty

Digoxigenin (DIG) labeling methods and kits for DNA and RNA DIG probes, random primed DNA labeling, nick translation labeling, 5’ and 3’ oligonucleotide end-labeling.

Metody znakowania digoksygeniną (DIG) i zestawy do sond DNA i RNA DIG, znakowanie DNA z losowym primerem, znakowanie nickiem translacyjnym, znakowanie końcowe oligonukleotydów 5' i 3'.

Nasz zespół naukowców ma doświadczenie we wszystkich obszarach badań, w tym w naukach przyrodniczych, materiałoznawstwie, syntezie chemicznej, chromatografii, analityce i wielu innych dziedzinach.

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