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Merck

11681460001

Roche

INT/BCIP Stock Solution

solution, pkg of 3 mL

Synonim(y):

5-bromo-4-chloro-3-indoyl phosphate, BCIP

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About This Item

Kod UNSPSC:
12352204

Postać

solution

masa cząsteczkowa

(INT: Mr = 505.7; BCIP toluidine salt: Mr = 433.6)

opakowanie

pkg of 3 mL

producent / nazwa handlowa

Roche

temp. przechowywania

2-8°C

Opis ogólny

BCIP (5-bromo-4-chloro-3-indoyl phosphate) is the AP (alkaline phosphatase)-substrate which after dephosphorylation reacts further to give a dark-blue indigo dye as an oxidation product. INT (iodonitrotetrazolium) is the oxidant producing a red dye. The reaction product has a reddish-brown color and is insoluble in water.

Zastosowanie

INT/BCIP Stock Solution is used for the sensitive detection of alkaline phosphatase (AP) in blotting protocols, that includes:
  • Southern blot
  • Western blot
  • immunohistochemistry and immunocytochemistry
It has been used for colorogenic in situ hybridization.

Charakterystyka techniczna

Formulas: INT: C19H13CIN5O2; BCIP: C8H6NO4BrCIP x C7H9N

Zasada

BCIP is the AP-substrate which after dephosphorylation reacts further to give a dark-blue indigo dye as an oxidation product. INT is the oxidant producing a red dye.

Postać fizyczna

Solution of 33 mg/ml INT (2-[4-iodophenyl]-3-[4-nitrophenyl]-5-phenyltetrazolium chloride) and 33 mg/ml BCIP (5-bromo-4-chloro-3-indolyl-phosphate, toluidine-salt in DMSO)

Uwaga dotycząca przygotowania

Working solution: Preparation of 10 ml Staining Solution
Bring the stock solution to 15 to 25 °C until all components are dissolved.
Add 75 μl of the stock solution to 10 ml 0.1 M Tris-buffer, pH 9.5, 0.05 M MgCl2, 0.1 M NaCl.
Note: Prepare the staining solution shortly before use.
Preparation of Additional Solutions Required
Blocking solution: Dissolve 0.5 g Blocking Reagent in 100 ml TBS, pH 7.5, by heating to 50 to 60 °C (1 hour). Dissolving the components can be accelerated by sonication or by incubation in a microwave oven.
Information Note: The solution remains turbid.

Inne uwagi

For life science research only. Not for use in diagnostic procedures.
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Kod klasy składowania

12 - Non Combustible Liquids

Klasa zagrożenia wodnego (WGK)

WGK 2

Temperatura zapłonu (°F)

does not flash

Temperatura zapłonu (°C)

does not flash


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