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Merck

11207733910

Roche

Anty-Digoxigenin-POD, fragmenty Fab

from sheep

Synonim(y):

antydigoksygenina, digoksygenina

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About This Item

Kod UNSPSC:
12352203

pochodzenie biologiczne

sheep

Poziom jakości

białko sprzężone

peroxidase conjugate

forma przeciwciała

F(ab′)2 fragment of affinity isolated antibody

rodzaj przeciwciała

primary antibodies

klon

polyclonal

Formularz

lyophilized (clear, brown solution after reconstitution)

opakowanie

pkg of 150 U

producent / nazwa handlowa

Roche

temp. przechowywania

2-8°C

Opis ogólny

Digoxigenin is a hapten, useful in labeling nucleic acids and in detection systems. This product contains Fab fragments from an anti-digoxigenin antibody, conjugated with horse-radish peroxidase (POD). Anti-Digoxigenin-POD, Fab fragments is useful for the detection of digoxigenin-labeled compounds.

Specyficzność

The polyclonal antibody from sheep is specific to digoxigenin and digoxin and shows no cross-reactivity with other steroids, such as human estrogens and androgens.

Zastosowanie

Use Anti-Digoxigenin-POD, Fab fragments for the detection of digoxigenin-labeled compounds using:
  • Dot blot
  • ELISA
  • Immunohistocytochemistry
  • In situ hybridization
  • Western blot

Uwaga dotycząca przygotowania

After immunization with digoxigenin, sheep IgG was purified by ion-exchange chromatography, and the specific IgG was isolated by immunosorption. The Fab fragments obtained by papain digestion were purified by gel filtration, conjugated to the specific label, and stabilized in buffer.

Working concentration: Working concentration of conjugate will depend on the application and substrate. The following concentrations should be taken as a guideline: Dot blot: 150 mU/ml
  • ELISA: 50 to 150 mU/ml
  • Immunohistocytochemistry: 250 to 500 mU/ml
  • In situ hybridization: 1.5 to 7.5 U/ml
  • Southern blot: 150 mU/ml
  • Western blot: 250 to 500 mU/ml

Working solution: 100 mM Tris-HCl, 150 mM NaCl, pH 7.5.
1% Blocking reagent (w/v), 1 to 5% heat inactivated fetal calf serum (v/v) or sheep normal serum can be used for reduction of unspecific binding.

Rekonstytucja

Add 1 ml double-distilled water to a final concentration of 150 U/ml.

Inne uwagi

For life science research only. Not for use in diagnostic procedures.
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Piktogramy

Exclamation mark

Hasło ostrzegawcze

Warning

Zwroty wskazujące rodzaj zagrożenia

Zwroty wskazujące środki ostrożności

Klasyfikacja zagrożeń

Skin Sens. 1

Kod klasy składowania

11 - Combustible Solids

Klasa zagrożenia wodnego (WGK)

WGK 1

Temperatura zapłonu (°F)

does not flash

Temperatura zapłonu (°C)

does not flash


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Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.

Odwiedź Bibliotekę dokumentów

Mamiko Yajima et al.
Developmental dynamics : an official publication of the American Association of Anatomists, 242(2), 155-163 (2012-11-23)
Meiosis is a unique mechanism in gamete production and a fundamental process shared by all sexually reproducing eukaryotes. Meiosis requires several specialized and highly conserved genes whose expression can also identify the germ cells undergoing gametogenic differentiation. Sea urchins are
Juha Kotimaa et al.
Molecular immunology, 76, 13-21 (2016-06-24)
Experimental mouse models have been extensively used to elucidate the role of the complement system in different diseases and injuries. Contribution of gender has revealed an intriguing gender specific difference; female mice often show protection against most complement driven injuries
Yutaka Daido et al.
Developmental biology, 392(1), 117-129 (2014-05-07)
The vertebrate retina contains two types of photoreceptor cells, rods and cones, which use distinct types of opsins and phototransduction proteins. Cones can be further divided into several subtypes with differing wavelength sensitivity and morphology. Although photoreceptor development has been
Sarah E Mercer et al.
PloS one, 7(12), e52375-e52375 (2013-01-10)
The inability to functionally repair tissues that are lost as a consequence of disease or injury remains a significant challenge for regenerative medicine. The molecular and cellular processes involved in complete restoration of tissue architecture and function are expected to
X Peng et al.
Leukemia, 29(12), 2355-2365 (2015-06-25)
Controlled self-renewal and differentiation of hematopoietic stem/progenitor cells (HSPCs) are critical for vertebrate development and survival. These processes are tightly regulated by the transcription factors, signaling molecules and epigenetic factors. Impaired regulations of their function could result in hematological malignancies.

Produkty

Digoxigenin (DIG) labeling methods and kits for DNA and RNA DIG probes, random primed DNA labeling, nick translation labeling, 5’ and 3’ oligonucleotide end-labeling.

Metody znakowania digoksygeniną (DIG) i zestawy do sond DNA i RNA DIG, znakowanie DNA z losowym primerem, znakowanie nickiem translacyjnym, znakowanie końcowe oligonukleotydów 5' i 3'.

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