Przejdź do zawartości
Merck
Wszystkie zdjęcia(3)

Key Documents

MECY2MAG-73KPX

Millipore

MILLIPLEX® Mouse Cytokine/Chemokine Magnetic Bead Panel II, MECY2MAG-73KPX

Simultaneously analyze multiple cytokine and chemokine biomarkers with Bead-Based Multiplex Assays using the Luminex technology, in mouse serum, plasma and cell culture samples.

Synonim(y):

Mouse Cytokine Assay

Zaloguj sięWyświetlanie cen organizacyjnych i kontraktowych


About This Item

Kod UNSPSC:
12161503
eCl@ss:
32161000
NACRES:
NA.47

Poziom jakości

reaktywność gatunkowa

mouse

producent / nazwa handlowa

Milliplex®

assay range

accuracy: 77-105%
standard curve range: 1-5,000 pg/mL
(IFNγ)

standard curve range: 12-50,000 pg/mL
(Exodus-2/CCL21/6Ckine)

standard curve range: 12-50,000 pg/mL
(IFNβ1)

standard curve range: 12-50,000 pg/mL
(IL-11)

standard curve range: 12-50,000 pg/mL
(IL-20)

standard curve range: 12-50,000 pg/mL
(MIP-3β/CCL19)

standard curve range: 2-10,000 pg/mL
(IL-16)

standard curve range: 2-10,000 pg/mL
(MCP-5/CCL12)

standard curve range: 2-10,000 pg/mL
(MDC/CCL22)

standard curve range: 2-10,000 pg/mL
(MIP-3α/CCL20)

standard curve range: 2-10,000 pg/mL
(TARC/CCL17)

standard curve range: 24-100,000 pg/mL
(Fractalkine/CX3CL1)

standard curve range: 24-100,000 pg/mL
(IL-17A/F)

standard curve range: 5-20,000 pg/mL
(Erythropoietin (EPO))

metody

multiplexing: suitable

kompatybilność

configured for Premixed

metoda wykrywania

fluorometric (Luminex xMAP)

Warunki transportu

wet ice

Opis ogólny

Cytokines are immunomodulatory polypeptides that play key roles in both adaptive and innate immune responses. A generic term, “cytokines” includes myokines (produced by muscle cells), lymphokines (produced by activated Th cells), interleukins (acting as mediators between T cells) and chemokines (responsible for T-cell migration). One of the regulatory mechanisms of the immune system, cytokines act at the recognition, activation, or effector phases of an immune response, modulating the development and functional activities of the subtypes of T cells, B cells and myeloid cells. Consequently, research involving cytokines plays a significant role in achieving a deeper understanding of the immune system and its multi-faceted response to most antigens, especially those responses that make up the inflammatory process. In addition, the mouse model and mouse-to-human translational studies provide data essential to understanding the role of cytokines and immune response in both normal and disease states.

The MILLIPLEX® Mouse Cytokine/Chemokine Bead Panel II Premixed 14 Plex, contains all the components necessary to measure the following 14 biomarkers using Luminex® xMAP® technology: Erythropoietin (EPO), Exodus-2/CCL21/6Ckine, Fractalkine/CX3CL1, IFNβ1, IFNγ, IL-11, IL-16, IL-17A/F, IL-20, MCP-5/CCL12, MDC/CCL22, MIP-3α/CCL20, MIP-3β/CCL19, TARC /CCL17. The kit uses a 96-well format, contains a lyophilized standard cocktail, two quality controls and can measure up to 38 serum, plasma, or cell/tissue culture samples in duplicate.

Panel Type: Cytokines/Chemokines

Specyficzność

There was no or negligible cross-reactivity between the antibodies for an analyte and any of the other analytes within a panel.

Zastosowanie

  • Analytes: Erythropoietin (EPO), Exodus-2, Fractalkine/CX3CL1, IFNβ1, IFNγ, IL-11, IL-16, IL-17A/F, IL-20, MCP-5/CCL12, MDC (CCL22), MIP-3α/CCL20, MIP-3β/CCL19, TARC/CCL17
  • Recommended Sample type: serum, plasma and cell culture
  • Recommended Sample dilution: 1:2 dilution of serum and plasma. Tissue culture supernatant may require a dilution with an appropriate control medium
  • Assay Run Time: overnight or one day
  • Research Category: Inflammation & Immunology

Opakowanie

Everything you need in a single kit.

Komponenty

Mouse Cytokine/Chemokine Panel II Standard, 1 vial, lyophilized

Mouse Cytokine/Chemokine Panel II Quality Control 1, 1 vial, lyophilized

Mouse Cytokine/Chemokine Panel II Quality Control 2, 1 vial, lyophilized

Serum Matrix (0.08% Sodium Azide), 1 vial, lyophylized

Assay Buffer, 1 bottle, 30 mL

Wash Buffer, 10X (0.05% Proclin), 2 bottles, 30 mL ea

Mouse Cytokine/Chemokine Panel II Detection Antibodies, 1 bottle, 3.2 mL

Mouse Cytokine/Chemokine Panel II Premixed 14-Plex Beads, 3.5 mL

Streptavidin-Phycoerythrin, 1 bottle, 3.2 mL

Mixing Bottle, 1 bottle

Sample Plate, 96 well format, 1 plate

2 Foil Plate Sealers

Przechowywanie i stabilność

Recommended storage for kit components is 2 - 8°C.

Inne uwagi

Please contact Technical Service for linearity of dilution.
Sensitivity: Refer to kit protocol for sensitivities of individual cytokines/chemokines.

Informacje prawne

Luminex is a registered trademark of Luminex Corp
MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany
xMAP is a registered trademark of Luminex Corp

Oświadczenie o zrzeczeniu się odpowiedzialności

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
This page may contain text that has been machine translated.

Piktogramy

Skull and crossbonesEnvironment

Hasło ostrzegawcze

Danger

Zwroty wskazujące rodzaj zagrożenia

Klasyfikacja zagrożeń

Acute Tox. 3 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Irrit. 2 - Skin Sens. 1

Kod klasy składowania

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

Klasa zagrożenia wodnego (WGK)

WGK 3


Certyfikaty analizy (CoA)

Poszukaj Certyfikaty analizy (CoA), wpisując numer partii/serii produktów. Numery serii i partii można znaleźć na etykiecie produktu po słowach „seria” lub „partia”.

Masz już ten produkt?

Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.

Odwiedź Bibliotekę dokumentów

Nasz zespół naukowców ma doświadczenie we wszystkich obszarach badań, w tym w naukach przyrodniczych, materiałoznawstwie, syntezie chemicznej, chromatografii, analityce i wielu innych dziedzinach.

Skontaktuj się z zespołem ds. pomocy technicznej