MAB3418

Anti-MAP2 Antibody

CHEMICON^®, mouse monoclonal, AP20

• Synonim(y): Microtubule Associated Protein, MAP2A and 2B
• Kod UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Właściwości

• Nazwa produktu: Anti-MAP2 Antibody, clone AP20, clone AP20, Chemicon^®, from mouse
• pochodzenie biologiczne: mouse
• Poziom jakości: 100
• forma przeciwciała: purified immunoglobulin
• rodzaj przeciwciała: primary antibodies
• klon: AP20, monoclonal
• reaktywność gatunkowa: bovine, mouse, rat, chicken, quail, Xenopus, human
• opakowanie: antibody small pack of 25 μg
• producent / nazwa handlowa: Chemicon^®
• metody: immunohistochemistry: suitable; western blot: suitable
• izotyp: IgG
• numer dostępu NCBI: NM_001039538.1
• numer dostępu UniProt: P11137
• Warunki transportu: ambient
• temp. przechowywania: 2-8°C
• docelowa modyfikacja potranslacyjna: unmodified
• informacje o genach: chicken ... Map2(424001) ; frog ... Map2(780043) ; human ... MAP2(4133) ; mouse ... Map2(17756) , Map2(281294) ; rat ... Map2(25595)

Opis

Opis ogólny

MAP-2 (microtubule associated protein-2) is one of several high molecular weight proteins that play an important role in brain neuron microtubule assembly. In addition to its association with microtubules, MAP-2 associates with neurofilaments and actin filaments suggesting that it may guide interaction among microtubules, other cytoskeletal elements, and cytoplasmic organelles. MAP-2 is a stringent marker for neurons. In addition, MAP-2 displays intracellular specificity. In the central nervous system, MAP-2 is confined to neuronal cell bodies and dendrites. There are exceptions, however, where some axons stain positive for small amounts of MAP-2. MAP-2 is uniformly distributed throughout the cell when first expressed in cultured neurons but becomes selectively localized as dendritic development proceeds. (Caceres, 1986; Dotti, 1987).

Specyficzność

Expected to cross-react with human, cow, chicken, quail, and Xenopus.

MAB3418 binds specifically to MAP-2.

Immunogen

Bovine brain microtubule protein.

Zastosowanie

Anti-MAP2 Antibody, clone AP20 detects level of MAP2 & has been published & validated for use in IH & WB.

Research Category
Neuroscience

Research Sub Category
Neuronal & Glial Markers

Western Blot:
60 ng/mL. In SDS-PAGE, MAP-2 from adult rat migrates as a closely associated doublet having a molecular weight of approximately 280-300 kD representing the MAP-2A and -2B isoforms but not the 70 kDa MAP-2C isoform. However, early in brain development (postnatal day 10 in rats), MAP-2 migrates as a single band that is identical to the lower molecular weight band of the adult MAP-2 doublet (MAP-2b). Later in development (postnatal days 17-18), the mobility of MAP-2 changes to the adult doublet form. (In the spinal cord, conversion to the adult form occurs earlier). Users should run 4%-20% SDS-PAGE gradient gels.

Immunohistochemistry:
5 µg/mL (4% PFA fixed frozen sections).

Immunohistochemistry:
5 µg/mL of a previous lot was used. Anti-MAP-2 can be used to stain tissue (brain or spinal cord) fixed with paraformaldehyde.

Optimal working concentrations must be determined by the end user.

Jakość

Routinely evaluated by Western Blot on Rat Brain lysate.

Western Blot Analysis:
1:1000 dilution of this lot detected MAP2 on 10 μg of Rat Brain lysate.

Opis wartości docelowych

230 kDa

Postać fizyczna

Format: Purified

Protein A purified

Purified mouse monoclonal IgG1 in buffer containing 0.02 M Phosphate buffer, pH 7.6, 0.25 M NaCl with 0.1% sodium azide.

Przechowywanie i stabilność

Stable for 6 months at 2-8°C in undiluted aliquots from date of receipt.
During shipment, small volumes of product will occasionally become entrapped in the seal of the product vial. For products with volumes of 200 µL or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container’s cap.

Komentarz do analizy

Control
Brain, Neuronal culture, Human glioblastoma T98G cells.

Inne uwagi

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Informacje prawne

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Oświadczenie o zrzeczeniu się odpowiedzialności

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Informacja o środkach bezpieczeństwa

• Kod klasy składowania: 12 - Non Combustible Liquids
• Klasa zagrożenia wodnego (WGK): WGK 1
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable