AB5622
Anti-MAP2 Antibody
CHEMICON®, rabbit polyclonal
Synonim(y):
Przeciwciało anty-MAP2
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About This Item
Kod UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Polecane produkty
Nazwa produktu
Anti-Microtubule-Associated Protein 2 (MAP2) Antibody, Chemicon®, from rabbit
pochodzenie biologiczne
rabbit
Poziom jakości
forma przeciwciała
saturated ammonium sulfate (SAS) precipitated
rodzaj przeciwciała
primary antibodies
klon
polyclonal
reaktywność gatunkowa
rat, mouse, human
producent / nazwa handlowa
Chemicon®
metody
ELISA: suitable
immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable
numer dostępu NCBI
numer dostępu UniProt
docelowa modyfikacja potranslacyjna
unmodified
informacje o genach
human ... MAP2(4133)
mouse ... Map2(17756)
rat ... Map2(25595)
Opis ogólny
The microtubule-associated protein 2 (MAP2) is an abundant neuronal cytoskeletal protein that binds to tubulin and stabilizes microtubules. (Herzog and Weber, 1978). MAP2 is essential for the development and maitenance of neuronal morphology (Matus, 1991). In neurons MAP2 occurs as three primary isoforms, the high molecular weight MAP2a, MAP2b, and the low molecular weight MAP2c, that result from alternative splicing of the MAP2 gene (Chung et al., 1996). The low molecular weight isoform, MAP2c, is expressed in developing brain and is down-regulated during brain maturation, whereas the high molecular weight MAP2b is expressed in both developing and adult brain. The MAP2a appears only after brain maturation (Tucker, 1990). All these forms bind to microtubules through a domain near the carboxyl terminus that contains either three or four similar repeats of a 31-amino-acid motif (Lewis et al., 1988). MAP2 together with MAP4 and tau proteins belong to the family of thermostable proteins associated with microtubules.
Specyficzność
Reaktywność przeciwciała anty-MAP2 z innymi gatunkami nie została określona. Przeciwciało anty-MAP2 jest specyficzne dla białka 2 związanego z mikrotubulami (MAP2). Przeciwciało rozpoznaje wszystkie izoformy MAP2 (MAP2A, MAP2B, MAP2C i MAP2D). Wykazuje największą immunoreaktywność z HMW-MAP2 (MAP2A i MAP2B).
Immunogen
Purified Microtubule-associated protein from rat brain.
Zastosowanie
Detect Microtubule-Associated Protein 2 (MAP2) using this Anti-Microtubule-Associated Protein 2 (MAP2) Antibody validated for use in ELISA, IC, IH, IH(P) & WB with more than 55 product citations.
Immunocytochemistry:
1:1,000 dilution of a previous lot was used on primary neurons.
Immunohistochemistry:
1:1,000 dilution on 4% paraformaldehyde / PBS fixed tissue sections.
Immunoblot:
1:2,000 dilution was used on a previous lot. Recognizes MAP2A (280-300kDa, and MAP2B (270-280KDa), and to a lesser extent MAP2C (70kDa) and MAP2D (70-75kDa).
ELISA:
greater than or equal to1:2,000 dilution of a previous lot was used.
Optimal working dilutions must be determined by the end user.
1:1,000 dilution of a previous lot was used on primary neurons.
Immunohistochemistry:
1:1,000 dilution on 4% paraformaldehyde / PBS fixed tissue sections.
Immunoblot:
1:2,000 dilution was used on a previous lot. Recognizes MAP2A (280-300kDa, and MAP2B (270-280KDa), and to a lesser extent MAP2C (70kDa) and MAP2D (70-75kDa).
ELISA:
greater than or equal to1:2,000 dilution of a previous lot was used.
Optimal working dilutions must be determined by the end user.
Research Category
Neuroscience
Neuroscience
Research Sub Category
Neuronal & Glial Markers
Neurofilament & Neuron Metabolism
Neuronal & Glial Markers
Neurofilament & Neuron Metabolism
Jakość
Immunohistochemistry(paraffin) Analysis:
MAP2 (cat. # AB5622) staining on Normal Cerebellum. Tissue pretreated with Citrate, pH 6.0. This lot of antibody was diluted to 1:500, using IHC-Select Detection with HRP-DAB. Immunoreactivity is seen as fiber-like- staining (brown) on microtubule.
Optimal Staining With TE Buffer, pH 9.0, Epitope Retrieval: Human Cerebellum
MAP2 (cat. # AB5622) staining on Normal Cerebellum. Tissue pretreated with Citrate, pH 6.0. This lot of antibody was diluted to 1:500, using IHC-Select Detection with HRP-DAB. Immunoreactivity is seen as fiber-like- staining (brown) on microtubule.
Optimal Staining With TE Buffer, pH 9.0, Epitope Retrieval: Human Cerebellum
Opis wartości docelowych
Approx. 280 kDa doublet (MAP2a and MAP2b) and approx. 70 kDa doublet (MAP2c) on SDS-PAGE.
Postać fizyczna
Ammonium Sulfate Precipitation
Format: Purified
Purified rabbit polyclonal in buffer containing PBS with 0.1% sodium azide as a preservative.
Przechowywanie i stabilność
Maintain at -20ºC in undiluted aliquots for up to 6 months after date of receipt. Avoid repeatedfreeze/thaw cycles.
Komentarz do analizy
Control
Human brain tissue or human glioblastoma T98G cells
Human brain tissue or human glioblastoma T98G cells
Informacje prawne
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Oświadczenie o zrzeczeniu się odpowiedzialności
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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polecane
Kod klasy składowania
12 - Non Combustible Liquids
Klasa zagrożenia wodnego (WGK)
nwg
Temperatura zapłonu (°F)
Not applicable
Temperatura zapłonu (°C)
Not applicable
Certyfikaty analizy (CoA)
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