MAB360
Anti-Glial Fibrillary Acidic Protein Antibody, clone GA5
ascites fluid, clone GA5, Chemicon®
Synonim(y):
GFAP
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About This Item
Kod UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Polecane produkty
pochodzenie biologiczne
mouse
Poziom jakości
forma przeciwciała
ascites fluid
rodzaj przeciwciała
primary antibodies
klon
GA5, monoclonal
reaktywność gatunkowa
rat, human, mouse, rabbit, chicken, pig, bovine
opakowanie
antibody small pack of 25 μL
producent / nazwa handlowa
Chemicon®
metody
immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable
izotyp
IgG1
numer dostępu NCBI
numer dostępu UniProt
Warunki transportu
ambient
temp. przechowywania
−20°C
docelowa modyfikacja potranslacyjna
unmodified
informacje o genach
human ... GFAP(2670)
Powiązane kategorie
Opis ogólny
Glial fibrillary acidic protein is a class-III intermediate filament. GFAP is the main constituent of intermediate filaments in astrocytes and serves as a cell specific marker that distinguishes differentiated astrocytes from other glial cells during the development of the central nervous system.
Specyficzność
Glial fibrillar acidic protein. On western blots of extracts from a human glioma cell line (U33CG/343MG), MAB360 recognizes a band at approximately 51 kDa corresponding to GFAP (Debus, 1983). By immunohistochemistry it recognizes astrocytes and Bergmann glia cells, glioma and glial cell derived tumors. Shows no cross-reactivity with vimentin.
Reactivity with other species has not been determined.
Immunogen
Purified GFAP from porcine spinal cord.
Zastosowanie
Anti-Glial Fibrillary Acidic Protein Antibody, clone GA5 detects level of Glial Fibrillary Acidic Protein & has been published & validated for use in IC, IH, IH(P) & WB with more than 65 product citations.
Immunocytochemistry:
A previous lot was used on cultured mammalian cells.
Immunohistochemistry(paraffin):
1:400-1:800 dilution from a previous lot was used on alcohol-fixed paraffin embedded sections of rat brain (cerebrum or cerebellum) and human brain.
Immunoblotting: A 1:1000 dilution of a previous lot was used.
Optimal working dilutions must be determined by the end user
A previous lot was used on cultured mammalian cells.
Immunohistochemistry(paraffin):
1:400-1:800 dilution from a previous lot was used on alcohol-fixed paraffin embedded sections of rat brain (cerebrum or cerebellum) and human brain.
Immunoblotting: A 1:1000 dilution of a previous lot was used.
Optimal working dilutions must be determined by the end user
Jakość
Routinely evaluated by Western Blot on Mouse brain lysates.
Western Blot Analysis:
1:1000 dilution of this lot detected GFAP on 10 μg of Mouse brain lysates.
Western Blot Analysis:
1:1000 dilution of this lot detected GFAP on 10 μg of Mouse brain lysates.
Opis wartości docelowych
~ 51 kDa
Powiązanie
Replaces: 04-1031; 04-1062
Postać fizyczna
Unpurified mouse monoclonal IgG1 liquid in buffer containing 15 mM sodium azide.
Komentarz do analizy
Control
Positive Control: Cultured astrocytes, brain tissue (white matter), spinal chord, retina
Positive Control: Cultured astrocytes, brain tissue (white matter), spinal chord, retina
Informacje prawne
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
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Kod klasy składowania
10 - Combustible liquids
Klasa zagrożenia wodnego (WGK)
WGK 1
Temperatura zapłonu (°F)
Not applicable
Temperatura zapłonu (°C)
Not applicable
Certyfikaty analizy (CoA)
Poszukaj Certyfikaty analizy (CoA), wpisując numer partii/serii produktów. Numery serii i partii można znaleźć na etykiecie produktu po słowach „seria” lub „partia”.
Masz już ten produkt?
Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.
O Nesic et al.
Neuroscience, 143(3), 779-792 (2006-11-01)
The effect of spinal cord injury (SCI) on the expression levels and distribution of water channel aquaporin 4 (AQP4) has not been studied. We have found AQP4 in gray and white matter astrocytes in both uninjured and injured rat spinal
Auto-catalytic ceria nanoparticles offer neuroprotection to adult rat spinal cord neurons.
Das, M; Patil, S; Bhargava, N; Kang, JF; Riedel, LM; Seal, S; Hickman, JJ
Biomaterials null
Expression of leptin and leptin receptor isoforms in the rat and human carotid body.
Porzionato A, Rucinski M, Macchi V, Stecco C, Castagliuolo I, Malendowicz LK, De Caro R
Brain Research null
Cellular organization of adult neurogenesis in the Common Marmoset.
Bunk, Eva C, et al.
Aging Cell, 10, 28-38 (2011)
L-M Chen et al.
Neuroscience, 151(2), 374-385 (2007-12-07)
NCBE (SLC4A10) is a member of the SLC4 family of bicarbonate transporters, several of which play important roles in intracellular-pH regulation and transepithelial HCO(3)(-) transport. Here we characterize a new antibody that was generated in rabbit against a fusion protein
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