647925

Trichostatin A

from Streptomyces sp., ≥98% (HPLC), lyophilized solid, HDAC inhibitor, Calbiochem^®

• Synonim(y): Kwas 4,6-dimetylo-7-[ p-dimetyloaminofenylo]-7-oksahepta-2,4-dienohydroksamowy, TSA, inhibitor HDAC IX
• Wzór empiryczny (zapis Hilla): C₁₇H₂₂N₂O₃
• Numer CAS: 58880-19-6
• Masa cząsteczkowa: 302.37
• Numer MDL: MFCD03848392
• Kod UNSPSC: 12352200
• NACRES: NA.77

Właściwości

• Nazwa produktu: Trichostatin A, Streptomyces sp., A potent and reversible, cell-permeable inhibitor of histone deacetylase.
• Poziom jakości: 100
• opis: RTECS - MI5215000
• Próba: ≥98% (HPLC)
• Formularz: lyophilized solid
• producent / nazwa handlowa: Calbiochem^®
• warunki przechowywania: OK to freeze
• kolor: off-white
• rozpuszczalność: ethanol: 1 mg/mL; DMSO: 20 mg/mL; methanol: soluble
• Warunki transportu: ambient
• temp. przechowywania: −20°C
• ciąg SMILES: N(O)C(=O)\C=C\C(=C\C(C)C(=O)c1ccc(cc1)N(C)C)\C
• InChI: 1S/C17H22N2O3/c1-12(5-10-16(20)18-22)11-13(2)17(21)14-6-8-15(9-7-14)19(3)4/h5-11,13,22H,1-4H3,(H,18,20)/b10-5+,12-11+
• Klucz InChI: RTKIYFITIVXBLE-WKWSCTOISA-N

Opis

Opis ogólny

A potent and reversible inhibitor of histone deacetylase. Blocks cell cycle progression at the G1 phase in HeLa cells and induces a 12-fold increase in intracellular levels of gelsolin. Induces reversion of oncogenic ras-tranformed NIH 3T3 cells to a normal morphology. Inhibits IL-2 gene expression (IC₅₀ = 73 nM) in Jurkat cells and shows immunosuppressive activity in a mouse model. IC₅₀ = 6 nM for HDAC1; 38 nM for HDAC4, and 8.6 nM for HDAC6A.

A potent and reversible, cell-permeable inhibitor of histone deacetylase. Blocks cell cycle progression at the G₁ phase in HeLa cells and induces a 12-fold increase in intracellular levels of gelsolin. Induces reversion of oncogenic ras-transformed NIH/3T3 cells to a normal morphology. Inhibits IL-2 gene expression (IC₅₀ = 73 nM) in Jurkat cells and shows immunosuppressive activity in a mouse model. Down-regulates p57^kip2 in Hep 3Bcells. IC₅₀ = 6 nM for HDAC1; 38 nM for HDAC4, and 8.6 nM for HDAC6A. 10 mM (500 µg/165 µl) solution of Trichostatin A, Streptomyces sp. (Cat. No. 647926) in DMSO is also available.

Działania biochem./fizjol.

Cell permeable: yes

Primary Target
histone deactylase

Product does not compete with ATP.

Reversible: yes

Target IC₅₀: 73 nM inhibiting IL-2 gene expressionin Jurkat cells

Ostrzeżenie

Toxicity: Harmful (C)

Rekonstytucja

Following reconstitution in DMSO, aliquot and freeze (-20°C). Following reconstitution in ethanol, refrigerate (4°C). DMSO stock solutions are stable for up to 6 months at -20°C. Ethanol stock solutions are stable for up to 3 months at 4°C.

Inne uwagi

Furumai, R., et al. 2001. Proc. Natl. Acad. Sci. USA98, 87.
Gray, S.G. and Ekstrom, T.J. 1998. Biochem. Biophys. Res. Commun. 245, 423.
Takahashi, I., et al. 1996. J. Antibiot. 49, 453.
Taunton, J., et al. 1996. Science 272, 408.
Futamura, M., et al. 1995. Oncogene 10, 1119.
Hoshikawa, Y., et al. 1994. Exp. Cell Res. 214, 189.

Informacje prawne

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

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Informacja o środkach bezpieczeństwa

• Kod klasy składowania: 10 - Combustible liquids
• Klasa zagrożenia wodnego (WGK): WGK 3