コンテンツへスキップ
Merck

HPLC Analysis of Isoleucine Enantiomers on Astec® CHIROBIOTIC® T (25 °C )

HPLC Analysis of Isoleucine Enantiomers on Astec® CHIROBIOTIC® T (25 °C ) application for HPLC

材料

ガードカートリッジ

製品番号
詳細
価格

Astec® CHIROBIOTIC® T Chiral HPLCガード

Cartridge, 5 μm particle size, L × I.D. 2 cm × 4 mm

分析カラム

製品番号
詳細
価格

Astec® CHIROBIOTIC® T Chiral HPLC Column

5 μm particle size, L × I.D. 25 cm × 4.6 mm

標準

製品番号
詳細
価格

L-イソロイシン

from non-animal source, meets EP, JP, USP testing specifications, suitable for cell culture, 98.5-101.0%

L-イソロイシン

reagent grade, ≥98% (HPLC)

D-イソロイシン

≥98% (TLC)

L-イソロイシン

L-イソロイシン

BioUltra, ≥99.5% (NT)

DL-Isoleucine

99%, FCC, FG

DL-Isoleucine

ReagentPlus®, 99%

L-イソロイシン

99%, FCC, FG

L-イソロイシン

certified reference material, TraceCERT®, Manufactured by: Sigma-Aldrich Production GmbH, Switzerland

CONDITIONS

column

Astec CHIROBIOTIC T, 25 cm x 4.6 mm I.D., 5 μm particles (12024AST)

mobile phase

[A] water; [B] methanol; [C] formic acid; (30:70:0.02, A:B:C)

flow rate

1 mL/min

column temp.

25 °C

detector

UV, 205 nm

injection

10 μL

sample

300 μg /mL in 30:70 water:methanol

詳細

法的情報

Astec is a registered trademark of Merck KGaA, Darmstadt, Germany
CHIROBIOTIC is a registered trademark of Sigma-Aldrich Co. LLC