Hydrolysis-Sensitive Dithiolethione Prodrug Micelles.

Hydrolysis-Sensitive Dithiolethione Prodrug Micelles.

Macromolecular bioscience (2015-06-24)

Urara Hasegawa, Naoya Tateishi, Hiroshi Uyama, André J van der Vlies

要旨

Prodrug micelles carrying 5-(4-hydroxyphenyl)-3H-1,2-dithiole-3-thione (ADT-OH), a compound possessing chemopreventive properties, are prepared from amphiphilic block copolymers linking ADT-OH via an ester bond using glycine (PAM-PGlyADT) and isoleucine linkers (PAM-PIleADT). The release of ADT-OH from the PAM-PIleADT micelles is much slower than the PAM-PGlyADT micelles. The PAM-PGlyADT micelles show comparable toxicity with ADT-OH in different cancer cell lines, whereas the PAM-PIleADT micelles are not toxic up to 400 µM. This ADT-ester prodrug micelle approach enables to modulate the release rate of ADT-OH and thus might find application in cancer therapy and prevention.

材料

製品番号

ブランド

製品内容

D2650

Sigma-Aldrich

ジメチルスルホキシド, Hybri-Max^™, sterile-filtered, BioReagent, suitable for hybridoma, ≥99.7%

472301

Sigma-Aldrich

ジメチルスルホキシド, ACS reagent, ≥99.9%

T6508

Sigma-Aldrich

トリフルオロ酢酸, ReagentPlus^®, 99%

D8418

Sigma-Aldrich

ジメチルスルホキシド, for molecular biology

179337

Sigma-Aldrich

メタノール, ACS reagent, ≥99.8%

319902

Sigma-Aldrich

酢酸エチル, ACS reagent, ≥99.5%

D65100

Sigma-Aldrich

ジクロロメタン, contains 40-150 ppm amylene as stabilizer, ACS reagent, ≥99.5%

319937

Sigma-Aldrich

N,N-ジメチルホルムアミド, ACS reagent, ≥99.8%

D2438

Sigma-Aldrich

ジメチルスルホキシド, sterile-filtered, BioPerformance Certified, meets EP, USP testing specifications, suitable for hybridoma

239313

Sigma-Aldrich

硫酸ナトリウム, ACS reagent, ≥99.0%, anhydrous, granular

C2432

Sigma-Aldrich

クロロホルム, contains 100-200 ppm amylenes as stabilizer, ≥99.5%

673811

Sigma-Aldrich

ジエチルエーテル, anhydrous, ACS reagent, ≥99.0%, contains BHT as inhibitor

D5879

Sigma-Aldrich

ジメチルスルホキシド, ReagentPlus^®, ≥99.5%

238597

Sigma-Aldrich

硫酸ナトリウム, ACS reagent, ≥99.0%, anhydrous, powder

346136

Sigma-Aldrich

ジエチルエーテル, ACS reagent, anhydrous, ≥99.0%, contains BHT as inhibitor

237205

Sigma-Aldrich

重炭酸カリウム, ACS reagent, 99.7%, powder, crystals or granules

91707

Sigma-Aldrich

トリフルオロ酢酸, puriss. p.a., suitable for HPLC, ≥99.0% (GC)

D4540

Sigma-Aldrich

ジメチルスルホキシド, ≥99.5% (GC), suitable for plant cell culture

199443

Sigma-Aldrich

酸化アルミニウム, activated, basic, Brockmann I

319988

Sigma-Aldrich

クロロホルム, contains ethanol as stabilizer, ACS reagent, ≥99.8%

360481

Sigma-Aldrich

1,4-ジオキサン, ACS reagent, ≥99.0%, contains ≤25 ppm BHT as stabilizer

M2128

Sigma-Aldrich

チアゾリルブルーテトラゾリウムブロミド, 98%

41640

Sigma-Aldrich

ジメチルスルホキシド, puriss. p.a., ACS reagent, ≥99.9% (GC)

M5655

Sigma-Aldrich

チアゾリルブルーテトラゾリウムブロミド, powder, BioReagent, suitable for cell culture, suitable for insect cell culture, ≥97.5% (HPLC)

199974

Sigma-Aldrich

酸化アルミニウム, activated, neutral, Brockmann I

107700

Sigma-Aldrich

4-(ジメチルアミノ)ピリジン, ReagentPlus^®, ≥99%

32213

Sigma-Aldrich

メタノール, puriss. p.a., ACS reagent, reag. ISO, reag. Ph. Eur., ≥99.8% (GC)

33120

Sigma-Aldrich

N,N-ジメチルホルムアミド, puriss. p.a., ACS reagent, reag. Ph. Eur., ≥99.8% (GC)

320269

Sigma-Aldrich

ジクロロメタン, ACS reagent, ≥99.5%, contains 40-150 ppm amylene as stabilizer

32222

Sigma-Aldrich

ジクロロメタン, puriss. p.a., ACS reagent, reag. ISO, ≥99.9% (GC)