SAE0021

Pyruvate Kinase M2 human

recombinant, expressed in E. coli, specific activity ≥100 unit/mg protein

• 別名: Cytosolic Thyroid hormone-binding protein (CTHBP), M2-PK, OPA-interacting protein 3 (OIP-3), PKM2, Pyruvate kinase 3 (PK3), Pyruvate kinase muscle isozyme, p58
• UNSPSCコード: 12352204
• NACRES: NA.54

特性

• リコンビナント: expressed in E. coli
• 品質水準: 200
• フォーム: lyophilized powder
• 比活性: ≥100 units/mg protein
• 輸送温度: ambient
• 保管温度: −20°C

詳細

詳細

Research area: Cell signaling This product is recombinant human PKM2 (P14618) expressed in E. coli and has a predicted molecular mass of 58 kDa. The embryonic pyruvate kinase M2 (PKM2) is one of the isoforms of pyruvate kinases (PK), found in mammals. It is coded by the PKM2 gene mapped to human chromosome 15q23. PKM2 is expressed in all cells excluding adult muscle, brain, and liver. This protein is usually present, as an active tetrameric form.

アプリケーション

Human pyruvate kinase M2 (PKM2) has been used to study the effects of phosphorylated PKM2 in inactivating myofibroblasts during renal fibrosis.PyruvateKinase M2 human has been used as an enzyme to study the inhibitory effect of a novel irreversible inhibitor on the kinaseactivity of PKM2. It has also been used in recombinant protein labelingand in pyruvate kinase enzyme activity assay.

生物化学的／生理学的作用

Pyruvate kinase (PK) is an important glycolytic enzyme catalyzing the last step of glycolysis, transferring a phosphate group from phosphoenolpyruvate to ADP to yield one molecule of ATP and one molecule of pyruvate. The embryonic pyruvate kinase M2 (PKM2) controls β-catenin transactivation. It plays an important role in aerobic glycolysis or the Warburg effect. PKM2 induces gene transcription and tumorigenesis.Pyruvate Kinase M2 (PKM2)plays a role in the regulation of cell growth, cell proliferation, andapoptosis. It is found predominantly in embryonic tissues and several humancancers. PKM2 acts as a cytosolic thyroid hormone binding protein(CTHBP) and as a phosphotyrosine binding protein. PKM2 binds tophosphotyrosine peptides which results in the release of its allostericactivator fructose-1,6-bisphosphate (FBP) is associated with the blockageof PKM2 activity.

単位の定義

One unit will convert 1.0 μmole of phosphoenol-pyruvate to pyruvate per minute at pH 7.6 at 37 °C, in the presence of 1 mM fructose-1,6-bisphosphate.

物理的形状

Supplied as a lyophilized powder containing phosphate buffer at pH7.5, NaCl, DTT and a carbohydrate stabilizer.

安全性情報

• 保管分類コード: 11 - Combustible Solids
• WGK: WGK 2
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable