コンテンツへスキップ
Merck
すべての画像(3)

Key Documents

View All Documentation

安全性情報

SAB4200740

Sigma-Aldrich

Anti-Neurofilament 160 antibody, Mouse monoclonal

clone NN18, purified from hybridoma cell culture

別名:

Anti-160 kDa neurofilament protein, Anti-NF-M, Anti-Neurofilament 3, Anti-Neurofilament triplet M protein

ログイン組織・契約価格を表示する
すべての画像(3)

About This Item

UNSPSCコード:
12352203
NACRES:
NA.41

由来生物

mouse

品質水準

200

抗体製品の状態

purified from hybridoma cell culture

抗体製品タイプ

primary antibodies

クローン

NN18, monoclonal

形状

buffered aqueous solution

分子量

antigen ~160 kDa

濃度

~1.0 mg/mL

テクニック

immunoblotting: 0.3-0.6 μg/mL using rat brain extract
immunofluorescence: 10-20 μg/mL using human bone marrow neuroblast SHSY5Y cell line
immunohistochemistry: 10-20 μg/mL using heat-retrieved formalin-fixed, paraffin-embedded human brain sections

アイソタイプ

IgG1

UniProtアクセッション番号

P07197

輸送温度

dry ice

保管温度

−20°C

ターゲットの翻訳後修飾

unmodified

詳細

Anti-Neurofilament 160 antibody, Mouse monoclonal, (mouse IgG1 isotype) is derived from the NN18 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from mouse immunized with neurofilaments purified from pig spinal cord. Neurofilaments (NFs) belong to the intermediate filament (IF) family and are expressed mainly in cells or tissues of neuronal origin. Three major NFs subunits were discovered: light (NF-L, ~68 kDa), medium (NF-M, ~160 kDa) and heavy (NF-H, ~200 kDa), which are themselves composed of two tetrameric protofilament complexes of monomeric proteins. Neurofilament 160, also known as NF-M or Neurofilament triplet M protein.

免疫原

neurofilaments purified from pig spinal cord

アプリケーション

Anti-Neurofilament 160 antibody has been used in immunoblotting, Immunohistochemistry, Immunofluorescence

生物化学的／生理学的作用

NFs can accumulate in large numbers within cell bodies and proximal axons of affected neurons in a variety of pathologies, including Charcot-Marie-Tooth disease (CMT), neurofilament inclusion disease (NFID), giant axonal neuropathy (GAN), diabetic neuropathy, spinal muscular atrophy (SMA) and spastic paraplegia. In addition, NFs accumulation were detected in Alzheimer′s (AD) and Parkinson′s disease (PD) patients. Elevated concentrations of Neurofilament 160 have been shown in cerebrospinal fluid (CSF) and serum samples from patients with brain injury.

物理的形状

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

適切な製品が見つかりませんか。  

製品選択ツール.をお試しください

保管分類コード

10 - Combustible liquids

引火点(°F)

Not applicable

引火点(℃)

Not applicable

適用法令

試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。

Jan Code

SAB4200740-100UL:
SAB4200740-VAR:
SAB4200740-BULK:

試験成績書（COA）

製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。

以前この製品を購入いただいたことがある場合

文書ライブラリで、最近購入した製品の文書を検索できます。

文書ライブラリにアクセスする

M M Black et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 8(9), 3296-3305 (1988-09-01)
The principal subunits of neurofilaments (NFs) of immature cultured sympathetic neurons have apparent Mr of 68,000 and 145,000; a 200,000 Mr subunit is also present, but at comparatively low levels. These subunits are referred to as the low (NFL), middle
G Shaw et al.
Brain research, 460(2), 227-234 (1988-09-20)
Previous immunocytochemical studies have shown immunological differences between neurofilaments in axons and those in dendrites and perikarya of many mature neuron types: it is now known that non-phosphorylated epitopes are normally seen in cell bodies and dendrites of mature neurons
M J Monteiro et al.
The Journal of cell biology, 108(2), 579-593 (1989-02-01)
We have used transient and stable DNA transfection to force synthesis of the mouse NF-L and NF-M genes in nonneuronal cultured animal cells. When the authentic NF-L gene (containing 1.7 kb of sequences 5' to the transcription initiation site) was
Eduardo Martínez-Morillo et al.
Clinical chemistry and laboratory medicine, 53(10), 1575-1584 (2015-02-27)
Brain injury is a medical emergency that needs to be diagnosed and treated promptly. Several proteins have been studied as biomarkers of this medical condition. The aims of this study were to: 1) evaluate the selectivity and precision of a
Elevated neurofilament levels in neurological diseases
Norgren N, et al.
Brain Research, 987(1), 25-31 (2003)
関連する文献をすべて表示

ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.

製品に関するお問い合わせはこちら（テクニカルサービス）