R3779

Anti-RbAp48/RbAp46 antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

• 別名: Anti-Retinoblastoma-binding protein
• MDL番号: MFCD02686588
• UNSPSCコード: 12352203
• NACRES: NA.41

特性

• 由来生物: rabbit
• 品質水準: 200
• 結合体: unconjugated
• 抗体製品の状態: IgG fraction of antiserum
• 抗体製品タイプ: primary antibodies
• クローン: polyclonal
• 形状: buffered aqueous solution
• 分子量: antigen 46 kDa (RbAp46); antigen 48 kDa (RbAp48)
• 化学種の反応性: human
• テクニック: indirect immunofluorescence: 1:500-1:1,000 using human HeLa cells fixed with paraformaldehyde-Triton; microarray: suitable; western blot: 1:2,000-1:4,000 using MCF7 nuclear extracts
• UniProtアクセッション番号: Q16576
• 輸送温度: dry ice
• 保管温度: −20°C
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... RBBP4(5928) , RBBP7(5931) ; mouse ... Rbbp4(19646) , Rbbp7(245688) ; rat ... Rbbp4(313048) , Rbbp7(83712)

詳細

詳細

pRB-associated proteins p48 (RbAp48) is a 425 amino acids WD-domain protein isolated as a RB1 (retinoblastoma binding protein). RbAp46 (also known as RBBP7- retinoblastoma-binding protein 7) has 89% amino acid identity with RbAp48.

免疫原

synthetic peptide corresponding to amino acids 413-425 of human RbAp48, conjugated to KLH via an N-terminal added lysine residue. The immunizing peptide is conserved in human, mouse, and rat.

アプリケーション

Anti-RbAp48/RbAp46 antibody produced in rabbit has been used in immunoblotting and immunofluorescence.

生物化学的／生理学的作用

RbAp46 and RbAp48 are two histone tail-targeting proteins, that are found in association with chromatin remodeling complexes Sin3A/ histone deacetylase (HDAC) and NURD (nucleosomal remodeling and deacetylation complex). Transcriptional repressors exert their effects by recruitment of the Sin3A/HDAC corepressor complex, which contains a module composed of Sin3A, HDAC1, HDAC2, RbAp46, RbAp48, SAP30, and others. In the NuRD complex, HDAC1, HDAC2, RbAp48, and RbAp46 associate with MTA2 (metastasis associated 1 family member 2), MBD3 (methyl-CpG binding domain protein), MAT1L1, MBD3L1 (methyl-CpG binding domain protein 3 like 1), CHD3, and CHD4 to form the nucleosomal remodeling and deacetylation (NuRD) complex.

物理的形状

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 10 - Combustible liquids
• WGK: WGK 3
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable
• 個人用保護具 (PPE): Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)