NA0800

GenElute ^™ HP セレクトプラスミド ギガプレップキット

sufficient for 5 preparations

• 別名: Gen Elute
• UNSPSCコード: 41105501
• NACRES: NA.52

特性

• 使用法: sufficient for 5 preparations
• 環境により配慮した代替製品の特徴: Designing Safer Chemicals; Learn more about the Principles of Green Chemistry.
• sustainability: Greener Alternative Product
• テクニック: DNA extraction: suitable
• 環境により配慮した代替製品カテゴリ: Aligned,
• 保管温度: 15-25°C

詳細

詳細

The GenElute HP Select Plasmid Gigaprep Kit offers a simple, rapid, and cost effective method for isolating endotoxin-free plasmid DNA from recombinant E. coli cultures. Up to 15 mg of high copy plasmid DNA with ≤0.1 endotoxin unit/μg plasmid DNA can be recovered from E. coli cultures in 1.2-2.5 L Luria Broth (LB) medium or 600 mL-1.2 L of Terrific Broth (TB) medium in less than 2 hours after harvesting the cells. Note that the actual yield depends on the strain, the plasmid, and the culture medium used.

An overnight recombinant E. coli culture is harvested by centrifugation and subjected to a modified alkaline-SDS lysis. The lysate is cleared by filtration followed by the addition of a binding solution optimized for endotoxin-free plasmid preparations. The plasmid DNA is then captured onto a silica membrane in the presence of high salts while endotoxins are prevented from adsorbing to the membrane. Contaminants are removed with three wash steps. Finally, the bound DNA is eluted in endotoxin-free water.

The DNA is ready for immediate use in applications such as restriction digest, cloning, sequencing, PCR, and transfection of endotoxin-sensitive cell lines.

We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has Inherently Safer Chemistry, compared to the standard use of phenol and chloroform to perform DNA extractions.

アプリケーション

GenElute^™ HP Select Plasmid Gigaprep Kit has been used to purify plasmids.

High-quality, endotoxin-free DNA is ready for immediate use for the most demanding applications including transfection with endotoxin-sensitive cells and gene therapy research.

特徴および利点

• Up to 15 mg with endotoxin levels of ≤ 0.1 EU/μg
• Only 90 minutes from pelleted cells to purified plasmid
• Vacuum format with no ethanol precipitation
• Can purify low-, medium-, and high-copy plasmid DNA

その他情報

詳細はwww.sigma-aldrich.com/genelutehpをご覧ください。

法的情報

GenElute is a trademark of Sigma-Aldrich Co. LLC

キットの構成要素は別途購入可能です。

• G3671 GenElute^™ HP Pro Gigaprep Binding Column

安全性情報

• ピクトグラム: GHS02,GHS08,GHS05,GHS07
• シグナルワード: Danger
• 危険有害性情報: H226,H290,H302,H315,H319,H334,H336,H372
• 注意書き: P210 - P233 - P234 - P301 + P312 - P303 + P361 + P353 - P305 + P351 + P338
• 危険有害性の分類: Acute Tox. 4 Oral - Eye Irrit. 2 - Flam. Liq. 3 - Met. Corr. 1 - Resp. Sens. 1 - Skin Irrit. 2 - STOT RE 1 Inhalation - STOT SE 3
• ターゲットの組織: Central nervous system, Lungs
• 保管分類コード: 3 - Flammable liquids
• 引火点(°F): 77.0 °F - closed cup
• 引火点(℃): 25 °C - closed cup