LIG1

DNAライゲーションキット

• UNSPSCコード: 12352204
• NACRES: NA.53

特性

• グレード: for molecular biology
• 品質水準: 200
• 使用法: kit sufficient for 150 ligation reactions
• 輸送温度: wet ice
• 保管温度: −20°C

詳細

詳細

Sigma′s DNA Ligation Kit contains all of the reagents necessary to perform DNA ligation reactions. Sticky-end ligations are more efficient than blunt-end ligations; these can be facilitated with the addition of PEG.

アプリケーション

Suitable for:

• Joining fragments of DNA into a cloning vector
• Mutagenesis
• Gene anyalysis and structure-function relationships

構成

Sufficient for 150 reactions:

• 300uL 10X Ligation Buffer (D2176) in 250 mM Tris-HCl (pH 7.8) 100 mM MgCl2, and 10 mM dithiothreitol
• 3 x 100 units T4 DNA Ligase (D2886) in 50% glycerol with 10 mM Tris-HCl (pH 7.5) 50 mM KCl, and 1 mM dithiothreitol
• 3 x 100 uL 10 mM ATP ( A3702)
• 50 uL Control pBR322 DNA, HAE III Digest (D9430) 0.5 ug/ul in 10 mM Tris-HCl (pH 8.0), and 1 mM EDTA
• 1.5 mL 24% (w/v) PEG Solution, (P 2454)
• 1.5 mL Molecular Biology Grade Water (W4502)

原理

One of the most important steps in the cloning process is the ligation of linear DNA into a cloning vector. DNA ligations are performed by incubating DNA fragments with appropriately linearized cloning vectors in the presence of buffer, ATP, and DNA ligase. Many parameters affect ligations such as the relative ratio of insert to vector, the quality and type of the DNA ends, the temperature of ligation and the concentration of DNA.

安全性情報

• ピクトグラム: GHS07
• シグナルワード: Warning
• 危険有害性情報: H319
• 注意書き: P264 - P280 - P305 + P351 + P338 - P337 + P313
• 危険有害性の分類: Eye Irrit. 2
• 保管分類コード: 10 - Combustible liquids
• WGK: WGK 3
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable