L8275

Lectin from Galanthus nivalis (snowdrop)

lyophilized powder

• 別名: Galanthus nivalis lectin, GNL
• MDL番号: MFCD00163718
• UNSPSCコード: 12352202
• NACRES: NA.32

特性

• フォーム: lyophilized powder
• 品質水準: 200
• 有効性: <20 μg per mL agglutination activity
• 組成: Protein, ≥90% Lowry
• 不純物: salt, essentially free
• 保管温度: 2-8°C

詳細

詳細

Lectin from Galanthus nivalis (snowdrop) is a tetrameric, 12 kDa mannose binding lectin. It belongs to the family, Amaryllidacea. It is specific to α-1,3- or 1,6- linked D-mannose of carbohydrates. Snowdrop lectins are toxic to insects.

アプリケーション

Lectin from Galanthus nivalis (snowdrop) has been used:

• to coat ELISA plates
• as reverse transcriptase inhibitor
• in ELISA for detection of envelope glycoproteins of the human and simian immunodeficiency viruses

生物化学的／生理学的作用

Lectin from Galanthus nivalis (snowdrop) provide resistance against pests and are used in transgenic plants. Snowdrop lectin functions as a carrier to transport fused proteins to the hemolymph as it crosses gut epithelium of target species to exhibit insecticidal effects.

GNL agglutinates rabbit erythrocytes; human erythrocytes are not agglutinated. GNL sugar specificity is directed to the nonreducing end of the terminal α-D-mannosyl residue of glycoconjugates.

調製ノート

Kakuの方法による、アフィニティ-クロマトグラフィ-精製品です。

アナリシスノート

凝集活性は、1 mg/mL溶液をPBS(pH 7.2)で段階希釈して測定し、μg/mLの単位で表します。凝集活性は、25°Cで1時間インキュベートした場合、ウサギ赤血球の2%懸濁液を凝集させる最低濃度で示されます。

安全性情報

• 保管分類コード: 11 - Combustible Solids
• WGK: WGK 3
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable
• 個人用保護具 (PPE): Eyeshields, Gloves, type N95 (US)