L2137

リポポリサッカリド from Salmonella enterica serotype minnesota

purified by gel-filtration chromatography

• 別名: LPS
• MDL番号: MFCD00131520
• UNSPSCコード: 12352201
• NACRES: NA.25

特性

• 由来生物: Salmonella enterica (Serotype minnesota)
• 品質水準: 200
• 形状: lyophilized powder
• 精製方法: gel-filtration chromatography
• 不純物: <1% Protein
• 色: white
• 溶解性: water: 4.90-5.10 mg/mL, cloudy to hazy, colorless
• 輸送温度: ambient
• 保管温度: 2-8°C

詳細

詳細

リポポリサッカライド（LPS）は、グラム陰性菌の細胞壁を特徴とする構成成分です。LPSとその脂質A部分は、一般的な病原体関連分子パターン（PAMP）を認識するトール様受容体タンパク質ファミリーのメンバーであるトール様受容体4（TLR4）によって、自然免疫系の細胞を刺激します。

This product is extracted from Salmonella Minnesota and purified by gel filtration. The source strain is ATCC 9700. Mechanisms of complement activation by LPS of both rough and smooth strains of Salmonella Minnesota were studied by immunoassay of the activation products.

アプリケーション

• TRPV4 is not the molecular sensor for bacterial lipopolysaccharides-induced calcium signaling: This study explores the signaling pathways activated by lipopolysaccharides from Salmonella enterica serotype Minnesota, demonstrating that TRPV4 is not involved, advancing understanding of immune responses to bacterial endotoxins (Wang et al., 2023).

生物化学的／生理学的作用

Lipopolysaccharides (LPS) are localized in the outer layer of the membrane and are, in noncapsulated strains, exposed on the cell surface. They contribute to the integrity of the outer membrane, and protect the cell against the action of bile salts and lipophilic antibiotics.

調製ノート

The product is soluble in water (5 mg/ml) or cell culture medium (1 mg/ml) yielding a hazy, faint yellow solution. A more concentrated, though still hazy, solution (20 mg/ml) has been achieved in aqueous saline after vortexing and warming to 70-80 ^oC. Lipopolysaccharides are molecules that form micelles in every solvent. Hazy solutions are observed in water and phosphate buffered saline. Organic solvents do not give clearer solutions. Methanol yields a turbid suspension with floaters, while water yields a homogeneously hazy solution.

その他情報

To gain a comprehensive understanding of our extensive range of Lipopolysaccharides for your research, we encourage you to visit our Carbohydrates Category page.

安全性情報

• 保管分類コード: 11 - Combustible Solids
• WGK: WGK 3
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable