H9286

抗アセチル-ヒストンH3 (Ac-Lys⁹) ウサギ宿主抗体

2-2.5 mg/mL, affinity isolated antibody, buffered aqueous solution

• 別名: Anti-H3K9ac
• MDL番号: MFCD02685921
• UNSPSCコード: 12352203
• NACRES: NA.41

特性

• 由来生物: rabbit
• 品質水準: 200
• 結合体: unconjugated
• 抗体製品の状態: affinity isolated antibody
• 抗体製品タイプ: primary antibodies
• クローン: polyclonal
• 形状: buffered aqueous solution
• 分子量: antigen ~17 kDa
• 化学種の反応性: bovine, human, Caenorhabditis elegans, frog, mouse, rat, Drosophila, chicken
• 濃度: 2-2.5 mg/mL
• テクニック: indirect immunofluorescence: 1:1,000 using NIH3T3 mouse fibroblast cell line treated with sodium butyrate; microarray: suitable; western blot: 1:1,000 using whole cell extract of the mouse fibroblast NIH3T3 cell line treated with sodium butyrate
• UniProtアクセッション番号: P68431
• 輸送温度: dry ice
• 保管温度: −20°C
• ターゲットの翻訳後修飾: acetylation (Lys9)
• 遺伝子情報: human ... H3F3A(3020) , H3F3B(3021) , HIST1H3A(8350) , HIST1H3B(8358) , HIST1H3C(8352) , HIST1H3D(8351) , HIST1H3E(8353) , HIST1H3F(8968) , HIST1H3G(8355) , HIST1H3H(8357) , HIST1H3I(8354) , HIST1H3J(8356) , HIST2H3A(333932) , HIST2H3C(126961) , HIST3H3(8290) ; mouse ... H3f3a(15078) , H3f3b(15081) , Hist1h3a(360198) , Hist1h3b(319150) , Hist1h3c(319148) , Hist1h3d(319149) , Hist1h3e(319151) , Hist1h3f(260423) , Hist1h3g(97908) , Hist1h3h(319152) , Hist1h3i(319153) , Hist2h3b(319154) , Hist2h3c1(15077) , Hist2h3c2(97114) ; rat ... H3f3b(117056)

詳細

詳細

Anti-acetyl-Histone H3 (Ac-Lys9) antibody produced in rabbit is suitable for:

• indirect immunofluorescence at a dilution of 1:1,000 using NIH3T3 mouse fibroblast cell line treated with sodium butyrate
• microarray
• western blot at a dilution of 1:1,000 using whole cell extract of the mouse fibroblast NIH3T3 cell line treated with sodium butyrate

免疫原

ヒトヒストンH3のN末端に相当する合成[Ac-Lys⁹]アセチル化ヒストンH3ペプチド(アミノ酸7-20)。この配列はマウス, ラット, ウシ, ニワトリ, カエル, Drosophila, C. elegansなど多くの生物種で同一であり、TetrahymenaヒストンH3でも高度に保存されている(1アミノ酸置換)。

アプリケーション

Anti-acetyl-Histone H3 (Ac-Lys9) antibody produced in rabbit has been used in:

• indirect immunofluorescence
• immunoblotting
• immunohistochemistry

生物化学的／生理学的作用

Histone proteins H3, H4, H2A, and H2B function as building blocks to package eukaryotic DNA into repeating nucleosome units that are folded in higher order chromatin fibers.

In most species, histone H3 is primarily acetylated at lysine 9, 14, 18, and 23. Acetylation at lysine 9 significantly influences histone deposition and chromatin assembly in some organisms. Acetylation of specific lysine residues in H3 is also associated with processes apart from transcription.

物理的形状

0.01M PBS溶液 (pH 7.4, 15mMアジ化ナトリウム含有)。

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 12 - Non Combustible Liquids
• WGK: nwg
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable