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包装
pack of 1 ea
メーカー／製品名
Cytiva RPN3000
保管温度
2-8°C
詳細
ECL™ Direct Nucleic Acid Labeling and Detection Systems are based on the direct labeling of DNA or RNA probes with horseradish peroxidase (HRP) in a simple 20 min chemical reaction. The resulting probe can be used without purification. Detection is achieved by generation of light via the HRP-catalyzed breakdown of luminol.
Each system includes the following reagents, sufficient for labeling 5 to 10 μg nucleic acid and detecting 2000 to 4000 cm2 of membrane (depending on product ordered): labeling reagent, crosslinker, control DNA, blocking agent, ECL™ Detection Reagents, and ECL™ Gold Hybridization Buffer.
Each system includes the following reagents, sufficient for labeling 5 to 10 μg nucleic acid and detecting 2000 to 4000 cm2 of membrane (depending on product ordered): labeling reagent, crosslinker, control DNA, blocking agent, ECL™ Detection Reagents, and ECL™ Gold Hybridization Buffer.
アプリケーション
ECL™ Direct Nucleic Acid has been used in Western blot, southern blot and hybridization.
特徴および利点
- Direct probe labeling in a 10 min reaction, 1 h from hybridization to detection with ECL™ Direct, Hybond® N+, and Hyperfilm ECL.
- Eliminates handling, waste, and regulatory issues associated with the use of radioactivity.
- No need to strip blots before reprobing.
- For fast and easy detection of medium- to high-target amounts in applications such as colony/plaque screens, dot blots, and PCR product analyses.
- Consistent results combining strong signals with very Low backgrounds.
保管および安定性
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
アナリシスノート
この製品の分析証明書は、www.cytiva.com.
法的情報
ECL is a trademark of Cytiva
Hybond is a registered trademark of Cytiva
シグナルワード
Danger
保管分類コード
12 - Non Combustible Liquids
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
Kostyantyn V Dmytruk et al.
BMC biotechnology, 11, 58-58 (2011-05-27)
The detection and quantification of uric acid in human physiological fluids is of great importance in the diagnosis and therapy of patients suffering from a range of disorders associated with altered purine metabolism, most notably gout and hyperuricaemia. The fabrication
The detection and sequencing of a broad-host-range conjugative IncP-1β plasmid in an epidemic strain of Mycobacterium abscessus subsp. bolletii.
Leao SC, et al.
PLoS ONE, 8(4), e60746-e60746 (2013)
CRISPR-PCS Protocol for Chromosome Splitting and Splitting Event Detection in Saccharomyces cerevisiae.
Sasano Y and Harashima S
Bio-protocol, 7(10) (2017)
Elevated urinary level of vitamin D binding protein as a novel biomarker for diabetic nephropathy.
Tian XQ, et al.
Experimental and Therapeutic Medicine, 7(2), 411-416 (2014)
Transfer of antibiotic resistance marker genes between lactic acid bacteria in model rumen and plant environments.
Toomey N, et al.
Applied and Environmental Microbiology, 75(10), 3146-3152 (2009)
資料
Background and protocols describing the various methods used by molecular biologists to detect samples of protein or nucleic acids bound to membranes.
ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.
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