07-164-AF647

Anti-phospho H2A.X (Ser139) Antibody, Alexa Fluor^™ 647 Conjugate

from rabbit, ALEXA FLUOR^™ 647

• 別名: Histone H2A.X, Histone H2A.X, H2a/x
• UNSPSCコード: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

特性

• 由来生物: rabbit
• 品質水準: 100
• 結合体: ALEXA FLUOR^™ 647
• 抗体製品の状態: purified antibody
• 抗体製品タイプ: primary antibodies
• クローン: polyclonal
• 化学種の反応性: human
• 化学種の反応性（ホモロジーによる予測）: vertebrates (based on 100% sequence homology)
• テクニック: immunocytochemistry: suitable
• アイソタイプ: IgG
• NCBIアクセッション番号: NP_002096
• UniProtアクセッション番号: P16104
• 輸送温度: wet ice
• ターゲットの翻訳後修飾: phosphorylation (pSer139)
• 遺伝子情報: human ... ATP10D(57205) , H2AX(3014)

詳細

詳細

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. H2A.X, also known as H2AFX, is a variant of Histone H2A that is found in a subset of nucleosomes. H2A.X has a unique C-terminal tail that becomes phosphorylated following double-stranded DNA breaks following irradiation or apoptosis. Phosphorylation of H2A.X on Ser139 is one of the earliest markers for apoptosis and DNA damage.

特異性

Recognizes Histone H2A.X phosphorylated at Serine 139.

免疫原

Epitope: Phosphorylated Ser139

KLH-conjugated linear peptide corresponding to phospho H2A.X (Ser139).

アプリケーション

Research Category
エピジェネティクス及び核内機能分子

エピジェネティクス及び核内機能分子

Research Sub Category
ヒストン

エピジェネティクス

See data sheet for cat# 07-164, Anti-phospho H2A.X (Ser139) for additional Western blot, Immnoprecipitation, and ChIP-seq analysis performed using an unconjugated version of this antibody.

This AlexaFluor647 conjugated Anti-phospho Histone H2A.X antibody is validated for use in mmunocytochemistry for the detection of phospho-Histone H2A.X.

品質

Immunocytochemistry Analysis:

A 1:100 dilution of this antibody detected phospho Histone H2A.X (Ser139) in HeLa cells.
Alexa Fluor^™ is a registered trademark of Life Technologies.

ターゲットの説明

~15 kDa observed

物理的形状

Protein A purified

Purified conjugated rabbit polyclonal antibody in buffer containing PBS and 15 mg/ml BSA with 0.1% Sodium Azide.

保管および安定性

Stable for 1 year at 2-8°C from date of receipt.

その他情報

Concentration: Please refer to lot specific datasheet.

法的情報

ALEXA FLUOR is a trademark of Life Technologies

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 12 - Non Combustible Liquids
• WGK: WGK 2
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable